Team:Paris/July 24

From 2008.igem.org

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(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
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{| border="1"  
{| border="1"  
|align="center"|'''Name'''
|align="center"|'''Name'''
 +
|align="center"|'''BioBrick'''
|align="center"|'''Tube N°'''
|align="center"|'''Tube N°'''
|align="center"|'''Enzyme 1'''
|align="center"|'''Enzyme 1'''
|align="center"|'''Enzyme 2'''
|align="center"|'''Enzyme 2'''
|align="center"|'''Observation'''
|align="center"|'''Observation'''
-
|align="center"|'''Expected Size Matrix'''
+
|align="center"|'''Exp Size Matrix'''
-
|align="center"|'''Expected Size BioBrick'''
+
|align="center"|'''Exp Size BioBrick'''
-
|align="center"|'''Measured Size Matrix'''
+
|align="center"|'''Mea Size Matrix'''
-
|align="center"|'''Measured Size BioBrick'''
+
|align="center"|'''Mea Size BioBrick'''
|-
|-
! rowspan="5" style="background: #ccccff;" | D100
! rowspan="5" style="background: #ccccff;" | D100
 +
! rowspan="5" style="background: #ccccff;" | B0034
|align="center"|1
|align="center"|1
! rowspan="2"| XbaI
! rowspan="2"| XbaI
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|-
|-
! rowspan="2" style="background: #ccccff;" | D101
! rowspan="2" style="background: #ccccff;" | D101
 +
! rowspan="5" style="background: #ccccff;" | J23101
|align="center"|1
|align="center"|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
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|-
|-
! rowspan="2" style="background: #ccccff;" | D102
! rowspan="2" style="background: #ccccff;" | D102
 +
! rowspan="5" style="background: #ccccff;" | J23109
|align="center"|1
|align="center"|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
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|-
|-
! rowspan="2" style="background: #ccccff;" | D103
! rowspan="2" style="background: #ccccff;" | D103
 +
! rowspan="5" style="background: #ccccff;" | R0079
|align="center"|1
|align="center"|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
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|-
|-
! rowspan="2" style="background: #ccccff;" | D104
! rowspan="2" style="background: #ccccff;" | D104
 +
! rowspan="5" style="background: #ccccff;" | R0040
|align="center"|1
|align="center"|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
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|-
|-
! rowspan="2" style="background: #ccccff;" | D105
! rowspan="2" style="background: #ccccff;" | D105
 +
! rowspan="5" style="background: #ccccff;" | S03154
|align="center"|1
|align="center"|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
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|-
|-
|align="center"|2
|align="center"|2
 +
! rowspan="2"| XbaI
 +
! rowspan="2"| PstI
 +
! rowspan="2"|
 +
! rowspan="2"|  pb
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! rowspan="2"|  pb
 +
|align="center"|
 +
|align="center"|
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|-
 +
|align="center"|3
 +
|align="center"|
 +
|align="center"|
 +
|-
 +
|align="center"|4
 +
| align="center"|EcoRI
 +
| align="center"|SpeI
 +
| align="center"|
 +
| align="center"|pb
 +
| align="center"| pb
|align="center"|
|align="center"|
|align="center"|
|align="center"|

Revision as of 18:01, 24 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enzyme 1 Enzyme 2 Observation Exp Size Matrix Exp Size BioBrick Mea Size Matrix Mea Size BioBrick
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
2
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI pb pb
3
4 EcoRI SpeI pb pb
D106 1 SpeI PstI BV pb 14 pb
2 XbaI PstI pb pb
3
4 EcoRI SpeI pb pb
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3
Retrieved from "http://2008.igem.org/Team:Paris/July_24"