Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)
(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
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{| border="1"  
{| border="1"  
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|align="center"|'''Name'''
+
|align=center|'''Name'''
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|align="center"|'''BioBrick'''
+
|align=center|'''BioBrick'''
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|align="center"|'''Tube N°'''
+
|align=center|'''Tube N°'''
-
|align="center"|'''Enz 1'''
+
|align=center|'''Enz 1'''
-
|align="center"|'''Enz 2'''
+
|align=center|'''Enz 2'''
-
|align="center"|'''Obs'''
+
|align=center|'''Obs'''
-
|align="center"|'''Exp Size Matrix'''
+
|align=center|'''Exp Size Matrix'''
-
|align="center"|'''Exp Size BB'''
+
|align=center|'''Exp Size BB'''
-
|align="center"|'''Mea Size Matrix'''
+
|align=center|'''Mea Size Matrix'''
-
|align="center"|'''Mea Size BB'''
+
|align=center|'''Mea Size BB'''
|-
|-
! rowspan="5" style="background: #ccccff;" | D100
! rowspan="5" style="background: #ccccff;" | D100
! rowspan="5" style="background: #ccccff;" | B0034
! rowspan="5" style="background: #ccccff;" | B0034
-
|align="center"|1
+
|align=center|1
! rowspan="2"| XbaI
! rowspan="2"| XbaI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 34 pb
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|align="center"|3
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|align=center|3
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|align="center"| EcoRI
+
|align=center| EcoRI
-
|align="center"| XbaI
+
|align=center| XbaI
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|align="center"| FV
+
|align=center| FV
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|align="center"| 2076 pb
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|align=center| 2076 pb
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|align="center"| 15 pb
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|align=center| 15 pb
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|align="center"|4
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! rowspan="2"| SpeI
! rowspan="2"| SpeI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2077 pb
! rowspan="2"| 14 pb
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! rowspan="2" style="background: #ccccff;" | D101
! rowspan="2" style="background: #ccccff;" | D101
! rowspan="2" style="background: #ccccff;" | J23101
! rowspan="2" style="background: #ccccff;" | J23101
-
|align="center"|1
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|align=center|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
! rowspan="2"| 883 pb
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! rowspan="1" style="background: #ccccff;" | D102
! rowspan="1" style="background: #ccccff;" | D102
! rowspan="1" style="background: #ccccff;" | J23109
! rowspan="1" style="background: #ccccff;" | J23109
-
|align="center"|1
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|align=center|1
! rowspan="1"| SpeI
! rowspan="1"| SpeI
! rowspan="1"| PstI
! rowspan="1"| PstI
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! rowspan="1"| 883 pb
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! rowspan="2" style="background: #ccccff;" | D103
! rowspan="2" style="background: #ccccff;" | D103
! rowspan="2" style="background: #ccccff;" | R0079
! rowspan="2" style="background: #ccccff;" | R0079
-
|align="center"|1
+
|align=center|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2222 pb
! rowspan="2"| 2222 pb
! rowspan="2"| 14 pb
! rowspan="2"| 14 pb
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! rowspan="2" style="background: #ccccff;" | D104
! rowspan="2" style="background: #ccccff;" | D104
! rowspan="2" style="background: #ccccff;" | R0040
! rowspan="2" style="background: #ccccff;" | R0040
-
|align="center"|1
+
|align=center|1
! rowspan="2"| SpeI
! rowspan="2"| SpeI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2119 pb
! rowspan="2"| 2119 pb
! rowspan="2"| 14 pb
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! rowspan="4" style="background: #ccccff;" | D105
! rowspan="4" style="background: #ccccff;" | D105
! rowspan="4" style="background: #ccccff;" | S03154
! rowspan="4" style="background: #ccccff;" | S03154
-
|align="center"|1
+
|align=center|1
! rowspan="1"| SpeI
! rowspan="1"| SpeI
! rowspan="1"| PstI
! rowspan="1"| PstI
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! rowspan="1"| 2750 pb
! rowspan="1"| 2750 pb
! rowspan="1"| 14 pb
! rowspan="1"| 14 pb
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|align="center"|
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|align=center|
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|align="center"|2
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|align=center|2
! rowspan="2"| XbaI
! rowspan="2"| XbaI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 707 pb
! rowspan="2"| 707 pb
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|align="center"|
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|align="center"|
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|align=center|
|-
|-
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|align="center"|4
+
|align=center|4
-
| align="center"|EcoRI
+
| align=center|EcoRI
-
| align="center"|SpeI
+
| align=center|SpeI
-
| align="center"|
+
| align=center|
-
| align="center"| 2056 pb
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| align=center| 2056 pb
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| align="center"| 708 pb
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| align=center| 708 pb
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|align="center"|
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|-
! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | S03879
! rowspan="4" style="background: #ccccff;" | S03879
-
|align="center"|1
+
|align=center|1
| align=center|SpeI
| align=center|SpeI
-
| align="center"|PstI
+
| align=center|PstI
-
| align="center"|BV
+
| align=center|BV
-
| align="center"| 2768 pb
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| align=center| 2768 pb
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| align="center"|14 pb
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| align=center|14 pb
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|align="center"|2
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|align=center|2
! rowspan="2"| XbaI
! rowspan="2"| XbaI
! rowspan="2"| PstI
! rowspan="2"| PstI
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! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 725 pb
! rowspan="2"| 725 pb
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|align=center|
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|align="center"|
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|-
|-
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|align="center"|4
+
|align=center|4
-
| align="center"|EcoRI
+
| align=center|EcoRI
-
| align="center"|SpeI
+
| align=center|SpeI
-
| align="center"|
+
| align=center|
-
| align="center"| 2756 pb
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| align=center| 2756 pb
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| align="center"| 726 pb
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| align=center| 726 pb
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! rowspan="2" style="background: #ccccff;" |D107
! rowspan="2" style="background: #ccccff;" |D107
! rowspan="2" style="background: #ccccff;" |C0079
! rowspan="2" style="background: #ccccff;" |C0079
-
|align="center"| 1
+
|align=center| 1
-
|align="center"|EcoRI
+
|align=center|EcoRI
-
|align="center"|SpeI
+
|align=center|SpeI
-
|align="center"|FI
+
|align=center|FI
-
|align="center"| 4402 pb
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|align=center| 4402 pb
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|align="center"| 779 pb
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|align="center"| 2
+
|align=center| 2
-
|align="center"|XbaI
+
|align=center|XbaI
-
|align="center"|PstI
+
|align=center|PstI
-
|align="center"|BI
+
|align=center|BI
-
|align="center"| 4003 pb
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|align=center| 4003 pb
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|align="center"| 778 pb
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|align=center| 778 pb
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|align=center|
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|-
! rowspan="2" style="background: #ccccff;"|D108
! rowspan="2" style="background: #ccccff;"|D108
! rowspan="2" style="background: #ccccff;"|C0179
! rowspan="2" style="background: #ccccff;"|C0179
-
|align="center"| 1
+
|align=center| 1
-
|align="center"| EcoRI
+
|align=center| EcoRI
-
|align="center"| SpeI
+
|align=center| SpeI
-
|align="center"| FI
+
|align=center| FI
-
|align="center"| 4402 pb
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|align=center| 4402 pb
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|align="center"| 746 pb
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|align=center| 746 pb
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|align="center"|
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|align="center"| 2
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|align=center| 2
-
|align="center"| XbaI
+
|align=center| XbaI
-
|align="center"| PstI
+
|align=center| PstI
-
|align="center"| BI
+
|align=center| BI
-
|align="center"| 4403 pb
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|align=center| 4403 pb
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|align="center"| 745 pb
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|align=center| 745 pb
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|align="center"|
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|align=center|
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|-
! rowspan="2" style="background: #ccccff;"|D109
! rowspan="2" style="background: #ccccff;"|D109
! rowspan="2" style="background: #ccccff;"|E0030
! rowspan="2" style="background: #ccccff;"|E0030
-
|align="center"| 1
+
|align=center| 1
-
|align="center"|EcoRI
+
|align=center|EcoRI
-
|align="center"|SpeI
+
|align=center|SpeI
-
|align="center"|FI
+
|align=center|FI
-
|align="center"| 3166 pb
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|align=center| 3166 pb
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|align="center"| 746 pb
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|align="center"| 2
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|align=center| 2
-
|align="center"| XbaI
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|align=center| XbaI
-
|align="center"| PstI
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|align=center| PstI
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|align="center"| BI
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|align=center| BI
-
|align="center"| 3167 pb
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|align=center| 3167 pb
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|align="center"| 745 pb
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|-
! rowspan="2" style="background: #ccccff;"|D110
! rowspan="2" style="background: #ccccff;"|D110
! rowspan="2" style="background: #ccccff;"|E0040
! rowspan="2" style="background: #ccccff;"|E0040
-
|align="center"| 1
+
|align=center| 1
-
|align="center"| EcoRI
+
|align=center| EcoRI
-
|align="center"| SpeI
+
|align=center| SpeI
-
|align="center"| FI
+
|align=center| FI
-
|align="center"| 2056 pb
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|align=center| 2056 pb
-
|align="center"| 743 pb
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|align=center| 743 pb
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|align="center"| 2
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-
|align="center"| XbaI
+
|align=center| XbaI
-
|align="center"| PstI
+
|align=center| PstI
-
|align="center"| BI
+
|align=center| BI
-
|align="center"| 2057 pb
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|align=center| 2057 pb
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|align="center"| 742 pb
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! rowspan="2" style="background: #ccccff;"|D111
! rowspan="2" style="background: #ccccff;"|D111
! rowspan="2" style="background: #ccccff;"|E1010
! rowspan="2" style="background: #ccccff;"|E1010
-
|align="center"| 1
+
|align=center| 1
-
|align="center"| EcoRI
+
|align=center| EcoRI
-
|align="center"| SpeI
+
|align=center| SpeI
-
|align="center"| FI
+
|align=center| FI
-
|align="center"| 4402 pb
+
|align=center| 4402 pb
-
|align="center"| 704 pb
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|align=center| 704 pb
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|align="center"|
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|align="center"| 2
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|align=center| 2
-
|align="center"| XbaI
+
|align=center| XbaI
-
|align="center"| PstI
+
|align=center| PstI
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|align="center"| BI
+
|align=center| BI
-
|align="center"| 4403 pb
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|align=center| 4403 pb
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|align="center"| 703 pb
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! rowspan="1" style="background: #ccccff;"|D116
! rowspan="1" style="background: #ccccff;"|D116
! rowspan="1" style="background: #ccccff;"|J23100
! rowspan="1" style="background: #ccccff;"|J23100
-
|align="center"|1
+
|align=center|1
! rowspan="1"| SpeI
! rowspan="1"| SpeI
! rowspan="1"| PstI
! rowspan="1"| PstI
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! rowspan="1"| 2100 pb
! rowspan="1"| 2100 pb
! rowspan="1"| 883 pb
! rowspan="1"| 883 pb
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! rowspan="1" style="background: #ccccff;"|D117
! rowspan="1" style="background: #ccccff;"|D117
! rowspan="1" style="background: #ccccff;"|J23107
! rowspan="1" style="background: #ccccff;"|J23107
-
|align="center"|1
+
|align=center|1
! rowspan="1"| SpeI
! rowspan="1"| SpeI
! rowspan="1"| PstI
! rowspan="1"| PstI
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! rowspan="1"| 2100 pb
! rowspan="1"| 2100 pb
! rowspan="1"| 883 pb
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! rowspan="1" style="background: #ccccff;"|D118
! rowspan="1" style="background: #ccccff;"|D118
! rowspan="1" style="background: #ccccff;"|B0015
! rowspan="1" style="background: #ccccff;"|B0015
-
|align="center"|1
+
|align=center|1
-
|align="center"|EcoRI
+
|align=center|EcoRI
-
|align="center"|XbaI
+
|align=center|XbaI
! rowspan="1"| FV
! rowspan="1"| FV
! rowspan="1"| 3303 pb
! rowspan="1"| 3303 pb
! rowspan="1"| 15 pb
! rowspan="1"| 15 pb
-
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! rowspan="1" style="background: #ccccff;"|D119
! rowspan="1" style="background: #ccccff;"|D119
! rowspan="1" style="background: #ccccff;"|I0500
! rowspan="1" style="background: #ccccff;"|I0500
-
|align="center"|1
+
|align=center|1
-
|align="center"|SpeI
+
|align=center|SpeI
-
|align="center"|PstI
+
|align=center|PstI
! rowspan="1"| FV
! rowspan="1"| FV
! rowspan="1"| 5621 pb
! rowspan="1"| 5621 pb
! rowspan="1"| 14 pb
! rowspan="1"| 14 pb
-
|align="center"|
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! rowspan="5" style="background: #ccccff;" | D120
! rowspan="5" style="background: #ccccff;" | D120
! rowspan="5" style="background: #ccccff;" | B0030
! rowspan="5" style="background: #ccccff;" | B0030
-
|align="center"|1
+
|align=center|1
! rowspan="2"| XbaI
! rowspan="2"| XbaI
! rowspan="2"| PstI
! rowspan="2"| PstI
Line 582: Line 582:
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
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|align="center"|3
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|align=center|3
-
|align="center"| EcoRI
+
|align=center| EcoRI
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|align="center"| XbaI
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|align=center| XbaI
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|align="center"| FV
+
|align=center| FV
-
|align="center"| 2079 pb
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|align=center| 2079 pb
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|align="center"| 15 pb
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|align="center"|4
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! rowspan="2"| SpeI
! rowspan="2"| SpeI
! rowspan="2"| PstI
! rowspan="2"| PstI
Line 604: Line 604:
! rowspan="2"| 2080 pb
! rowspan="2"| 2080 pb
! rowspan="2"| 14 pb
! rowspan="2"| 14 pb
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! rowspan="1" style="background: #ccccff;"|D121
! rowspan="1" style="background: #ccccff;"|D121
! rowspan="1" style="background: #ccccff;"|E0422
! rowspan="1" style="background: #ccccff;"|E0422
-
|align="center"|1
+
|align=center|1
-
|align="center"|XbaI
+
|align=center|XbaI
-
|align="center"|PstI
+
|align=center|PstI
! rowspan="1"| FV
! rowspan="1"| FV
! rowspan="1"| 2057 pb
! rowspan="1"| 2057 pb
! rowspan="1"| 939 pb
! rowspan="1"| 939 pb
-
|align="center"|
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|-
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! rowspan="1" style="background: #ccccff;"|D122
+
! rowspan="1" "style=background: #ccccff;"|D122
! rowspan="1" style="background: #ccccff;"|E0840
! rowspan="1" style="background: #ccccff;"|E0840
-
|align="center"|1
+
|align=center|1
-
|align="center"|XbaI
+
|align=center|XbaI
-
|align="center"|PstI
+
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! rowspan="1"| FV
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Revision as of 19:23, 24 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 (=MP1) J23100
MP117 (=MP2) J23107
MP118 (=MP3) B0015
MP119 (=MP4) I0500
MP120 (=MP5) B0030
MP121 (=MP6) E0422
MP122 (=MP8) E0840



Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

list of digestion

Name Miniprep Biobrick Enzyme 1 Enzyme 2 Nber tube
D100 MP100 B0034 XbaI PstI 2
EcoRI XbaI 1
SpeI PstI 2
D101 MP101 J23101 SpeI PstI 2
D102 MP102 J23109 SpeI PstI 2
D103 MP103 R0079 SpeI PstI 2
D104 MP104 R0040 SpeI PstI 2
D105 MP105 S03154 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D106 MP106 J23101 SpeI PstI 1
XbaI PstI 2
EcoRI SpeI 1
D107 MP107 C0079 EcoRI SpeI 1
D108 MP108 C0179 EcoRI SpeI 1
D109 MP109 E0030 EcoRI SpeI 1
D110 MP110 E0040 EcoRI SpeI 1
D111 MP111 E1010 EcoRI SpeI 1
D116 MP116 J23100 SpeI PstI 2
D117 MP117 J23107 SpeI PstI 2
D118 MP118 B0015 EcoRI XbaI 2
D119 MP119 I0500 SpeI PstI 2
D120 MP120 B0030 SpeI PstI 2
EcoRI XbaI 1
XbaI PstI 2
D121 MP121 E0422 XbaI PstI 3
D122 MP122 E0840 XbaI PstI 3


Results of digestions : Electrophoresis

Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI 2057 pb 707 pb
3
4 EcoRI SpeI 2056 pb 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb
2 XbaI PstI 2057 pb 725 pb
3
4 EcoRI SpeI 2756 pb 726 pb
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb
2 XbaI PstI BI 4003 pb 778 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb
2 XbaI PstI BI 4403 pb 745 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb
2 XbaI PstI BI 3167 pb 745 pb
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb
2 XbaI PstI BI 2057 pb 742 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb
2 XbaI PstI BI 4403 pb 703 pb
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb
2
3 EcoRI XbaI FV 2079 pb 15 pb
4 SpeI PstI BV 2080 pb 14 pb
5
D121 E0422 1 XbaI PstI FV 2057 pb 939 pb
D122 E0840 1 XbaI PstI FV pb pb

gel1
gel1
gel2
gel2
gel3
gel3
gel4
gel4
Retrieved from "http://2008.igem.org/Team:Paris/July_24"