Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)
(→Results of digestions : Electrophoresis)
(→Results of digestions : Electrophoresis)
Line 94: Line 94:
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 34 pb
! rowspan="2"| 34 pb
-
! rowspan="2"| not digested
+
! rowspan="2"|  
-
! rowspan="2"| not digested
+
! rowspan="2"|  
-
! rowspan="2"| 8
+
! rowspan="2"|  
-
! rowspan="2"| 5-6
+
! rowspan="2"|  
|-
|-
|align=center|2
|align=center|2
Line 107: Line 107:
|align=center| 2076 pb
|align=center| 2076 pb
|align=center| 15 pb
|align=center| 15 pb
-
|align=center| 2100 pb
+
|align=center|
-
|align=center| -
+
|align=center|  
-
|align=center| 1
+
|align=center|  
-
|align=center| 2
+
|align=center|  
|-
|-
|align=center|4
|align=center|4
Line 118: Line 118:
! rowspan="2"| 2077 pb
! rowspan="2"| 2077 pb
! rowspan="2"| 14 pb
! rowspan="2"| 14 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| -
+
! rowspan="2"|  
-
|align=center| 1
+
|align=center|  
-
|align=center| 3-4
+
|align=center|  
|-
|-
|align=center|5
|align=center|5
-
|align=center| 1
+
|align=center|  
-
|align=center| 5-6
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;" | D101
! rowspan="2" style="background: #ccccff;" | D101
Line 135: Line 135:
! rowspan="2"| 2100 pb
! rowspan="2"| 2100 pb
! rowspan="2"| 883 pb
! rowspan="2"| 883 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| 900 pb
+
! rowspan="2"|  
-
|align=center| 1
+
|align=center|  
-
|align=center| 7-8
+
|align=center|  
|-
|-
|align=center|2
|align=center|2
-
|align=center| 1
+
|align=center|  
-
|align=center| 9-10
+
|align=center|  
|-
|-
! rowspan="1" style="background: #ccccff;" | D102
! rowspan="1" style="background: #ccccff;" | D102
Line 152: Line 152:
! rowspan="1"| 2100 pb
! rowspan="1"| 2100 pb
! rowspan="1"| 883 pb
! rowspan="1"| 883 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 900 pb
+
|align=center|  
-
|align=center| 1 & 2
+
|align=center|  
-
|align=center| 11-12 & 2-3
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;" | D103
! rowspan="2" style="background: #ccccff;" | D103
Line 165: Line 165:
! rowspan="2"| 2222 pb
! rowspan="2"| 2222 pb
! rowspan="2"| 14 pb
! rowspan="2"| 14 pb
-
! rowspan="2"| 2500 pb
+
! rowspan="2"|  
-
! rowspan="2"| -
+
! rowspan="2"|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 4-5
+
|align=center|  
|-
|-
|align=center|2
|align=center|2
-
|align=center| 2
+
|align=center|  
-
|align=center| 6-7
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;" | D104
! rowspan="2" style="background: #ccccff;" | D104
Line 182: Line 182:
! rowspan="2"| 2119 pb
! rowspan="2"| 2119 pb
! rowspan="2"| 14 pb
! rowspan="2"| 14 pb
-
! rowspan="2"| 2500 pb
+
! rowspan="2"|  
-
! rowspan="2"| -
+
! rowspan="2"|  
-
|align=center| 2
+
|align=center|  
-
|align=center| 8-9
+
|align=center|  
|-
|-
|align=center|2
|align=center|2
-
|align=center| 2
+
|align=center|  
-
|align=center| 10-11
+
|align=center|  
|-
|-
! rowspan="4" style="background: #ccccff;" | D105
! rowspan="4" style="background: #ccccff;" | D105
Line 199: Line 199:
! rowspan="1"| 2750 pb
! rowspan="1"| 2750 pb
! rowspan="1"| 14 pb
! rowspan="1"| 14 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| -
+
|align=center|  
-
|align=center| 2 & 3
+
|align=center|  
-
|align=center| 12 & 2
+
|align=center|  
|-
|-
|align=center|2
|align=center|2
Line 210: Line 210:
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 707 pb
! rowspan="2"| 707 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|
-
! rowspan="2"| 700 pb
+
! rowspan="2"|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 3-4
+
|align=center|  
|-
|-
|align=center|3
|align=center|3
-
|align=center| 3
+
|align=center|  
-
|align=center| 5-6
+
|align=center|  
|-
|-
|align=center|4
|align=center|4
Line 225: Line 225:
| align=center| 2056 pb
| align=center| 2056 pb
| align=center| 708 pb
| align=center| 708 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 7-8
+
|align=center|  
|-
|-
! rowspan="4" style="background: #ccccff;" | D106
! rowspan="4" style="background: #ccccff;" | D106
Line 238: Line 238:
| align=center| 2768 pb
| align=center| 2768 pb
| align=center|14 pb
| align=center|14 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| -
+
|align=center|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 9-10
+
|align=center|  
|-
|-
|align=center|2
|align=center|2
Line 249: Line 249:
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 725 pb
! rowspan="2"| 725 pb
-
! rowspan="2"| 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| 700 pb
+
! rowspan="2"|  
-
|align=center| 3
+
|align=center|  
-
|align=center| 11-12
+
|align=center|  
|-
|-
|align=center|3
|align=center|3
-
|align=center| 4
+
|align=center|  
-
|align=center| 2-3
+
|align=center|  
|-
|-
|align=center|4
|align=center|4
Line 264: Line 264:
| align=center| 2756 pb
| align=center| 2756 pb
| align=center| 726 pb
| align=center| 726 pb
-
|align=center| 2500 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 4
+
|align=center|  
-
|align=center| 4-5
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;" |D107
! rowspan="2" style="background: #ccccff;" |D107
Line 277: Line 277:
|align=center| 4402 pb
|align=center| 4402 pb
|align=center| 779 pb
|align=center| 779 pb
-
|align=center| 6000 pb
+
|align=center|  
-
|align=center| 1000 pb
+
|align=center|  
-
|align=center| 4
+
|align=center|  
-
|align=center| 6-7
+
|align=center|  
|-
|-
|align=center| 2
|align=center| 2
Line 288: Line 288:
|align=center| 4003 pb
|align=center| 4003 pb
|align=center| 778 pb
|align=center| 778 pb
-
|align=center| 5000 pb
+
|align=center|  
-
|align=center| 100 pb
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 9
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D108
! rowspan="2" style="background: #ccccff;"|D108
Line 301: Line 301:
|align=center| 4402 pb
|align=center| 4402 pb
|align=center| 746 pb
|align=center| 746 pb
-
|align=center| 2500 pb
+
|align=center|
-
|align=center| 1000 pb
+
|align=center|  
-
|align=center| 4
+
|align=center|  
-
|align=center| 8-9
+
|align=center|  
|-
|-
|align=center| 2
|align=center| 2
Line 312: Line 312:
|align=center| 4403 pb
|align=center| 4403 pb
|align=center| 745 pb
|align=center| 745 pb
-
|align=center| 2500 pb
+
|align=center|  
-
|align=center| 1000 pb
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 10
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D109
! rowspan="2" style="background: #ccccff;"|D109
Line 325: Line 325:
|align=center| 3166 pb
|align=center| 3166 pb
|align=center| 746 pb
|align=center| 746 pb
-
|align=center| 4000 pb
+
|align=center|  
-
|align=center| 1000 pb
+
|align=center|  
-
|align=center| 4
+
|align=center|  
-
|align=center| 10-11
+
|align=center|  
|-
|-
|align=center| 2
|align=center| 2
Line 336: Line 336:
|align=center| 3167 pb
|align=center| 3167 pb
|align=center| 745 pb
|align=center| 745 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| 1000 pb
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 11
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D110
! rowspan="2" style="background: #ccccff;"|D110
Line 349: Line 349:
|align=center| 2056 pb
|align=center| 2056 pb
|align=center| 743 pb
|align=center| 743 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 800 pb
+
|align=center|  
-
|align=center| 4 & 5
+
|align=center|  
-
|align=center| 12 & 2
+
|align=center|  
|-
|-
|align=center| 2
|align=center| 2
Line 360: Line 360:
|align=center| 2057 pb
|align=center| 2057 pb
|align=center| 742 pb
|align=center| 742 pb
-
|align=center| 2500 pb
+
|align=center|  
-
|align=center| 1000 pb
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 12
+
|align=center|  
|-
|-
! rowspan="2" style="background: #ccccff;"|D111
! rowspan="2" style="background: #ccccff;"|D111
Line 373: Line 373:
|align=center| 4402 pb
|align=center| 4402 pb
|align=center| 704 pb
|align=center| 704 pb
-
|align=center| 4000 pb
+
|align=center|  
-
|align=center| 600 pb
+
|align=center|  
-
|align=center| 5
+
|align=center|  
-
|align=center| 3-4
+
|align=center|  
|-
|-
|align=center| 2
|align=center| 2
Line 384: Line 384:
|align=center| 4403 pb
|align=center| 4403 pb
|align=center| 703 pb
|align=center| 703 pb
-
|align=center| 4500 pb
+
|align=center|  
-
|align=center| 700 pb
+
|align=center|  
-
|align=center| 8
+
|align=center|  
-
|align=center| 2-3
+
|align=center|  
|-
|-
-
! rowspan="1" style="background: #ccccff;"|D116
+
! rowspan="2" style="background: #ccccff;"|D116
-
! rowspan="1" style="background: #ccccff;"|J23100
+
! rowspan="2" style="background: #ccccff;"|J23100
|align=center|1
|align=center|1
-
! rowspan="1"| SpeI
+
! rowspan="2"| SpeI
-
! rowspan="1"| PstI
+
! rowspan="2"| PstI
-
! rowspan="1"| BV
+
! rowspan="2"| BV
-
! rowspan="1"| 2100 pb
+
! rowspan="2"| 2100 pb
-
! rowspan="1"| 883 pb
+
! rowspan="2"| 883 pb
-
! colspan="4"| Not realised
+
! colspan="4"|  
|align=center|
|align=center|
|-
|-
-
! rowspan="1" style="background: #ccccff;"|D117
+
|align=center|2
-
! rowspan="1" style="background: #ccccff;"|J23107
+
|
 +
|
 +
|-
 +
! rowspan="2" style="background: #ccccff;"|D117
 +
! rowspan="2" style="background: #ccccff;"|J23107
|align=center|1
|align=center|1
-
! rowspan="1"| SpeI
+
! rowspan="2"| SpeI
-
! rowspan="1"| PstI
+
! rowspan="2"| PstI
-
! rowspan="1"| BV
+
! rowspan="2"| BV
-
! rowspan="1"| 2100 pb
+
! rowspan="2"| 2100 pb
-
! rowspan="1"| 883 pb
+
! rowspan="2"| 883 pb
-
|align=center| 2000 pb
+
|align=center|  
-
|align=center| 800 pb
+
|align=center|
-
|align=center| 5
+
|align=center|
-
|align=center| 5-6
+
|align=center|
 +
|align=center|
|-
|-
-
! rowspan="1" style="background: #ccccff;"|D118
+
|align=center|2
-
! rowspan="1" style="background: #ccccff;"|B0015
+
|
 +
|-
 +
! rowspan="2" style="background: #ccccff;"|D118
 +
! rowspan="2" style="background: #ccccff;"|B0015
|align=center|1
|align=center|1
|align=center|EcoRI
|align=center|EcoRI
|align=center|XbaI
|align=center|XbaI
-
! rowspan="1"| FV
+
! rowspan="2"| FV
-
! rowspan="1"| 3303 pb
+
! rowspan="2"| 3303 pb
-
! rowspan="1"| 15 pb
+
! rowspan="2"| 15 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| -
+
|align=center|  
-
|align=center| 5
+
|align=center|  
-
|align=center| 7-8
+
|align=center|  
 +
|align=center|
 +
|-
 +
|align=center|2
 +
|
|-
|-
-
! rowspan="1" style="background: #ccccff;"|D119
+
! rowspan="2" style="background: #ccccff;"|D119
-
! rowspan="1" style="background: #ccccff;"|I0500
+
! rowspan="2" style="background: #ccccff;"|I0500
|align=center|1
|align=center|1
|align=center|SpeI
|align=center|SpeI
|align=center|PstI
|align=center|PstI
-
! rowspan="1"| FV
+
! rowspan="2"| FV
-
! rowspan="1"| 5621 pb
+
! rowspan="2"| 5621 pb
-
! rowspan="1"| 14 pb
+
! rowspan="2"| 14 pb
-
|align=center| 6000 - 3000 pb
+
! rowspan="2"| 
-
|align=center| -
+
|align=center|
-
|align=center| 5
+
|align=center|
-
|align=center| 9-10
+
|align=center|
 +
|align=center|
 +
|-
 +
|align=center|2
 +
|
 +
|
|-
|-
! rowspan="5" style="background: #ccccff;" | D120
! rowspan="5" style="background: #ccccff;" | D120
Line 447: Line 464:
! rowspan="2"| 2057 pb
! rowspan="2"| 2057 pb
! rowspan="2"| 37 pb
! rowspan="2"| 37 pb
-
! rowspan="2"| not digested
+
! rowspan="2"|
-
! rowspan="2"| not digested
+
! rowspan="2"|
-
|align=center| 7
+
|align=center|
-
|align=center| 2
+
|align=center|  
|-
|-
|align=center|2
|align=center|2
-
|align=center| 7
+
|align=center|  
-
|align=center| 5-6
+
|align=center|  
|-
|-
|align=center|3
|align=center|3
Line 462: Line 479:
|align=center| 2079 pb
|align=center| 2079 pb
|align=center| 15 pb
|align=center| 15 pb
-
|align=center| 1600 pb
+
|align=center|  
-
|align=center| -
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 2
+
|align=center|  
|-
|-
|align=center|4
|align=center|4
Line 473: Line 490:
! rowspan="2"| 2080 pb
! rowspan="2"| 2080 pb
! rowspan="2"| 14 pb
! rowspan="2"| 14 pb
-
! rowspan="2"| 3000 - 2000 pb
+
! rowspan="2"|  
-
! rowspan="2"| -
+
! rowspan="2"|  
-
|align=center| 5
+
|align=center|  
-
|align=center| 11
+
|align=center|  
|-
|-
|align=center|5
|align=center|5
-
|align=center| 5
+
|align=center|  
-
|align=center| 12
+
|align=center|  
|-
|-
! rowspan="1" style="background: #ccccff;"|D121
! rowspan="1" style="background: #ccccff;"|D121
Line 487: Line 504:
|align=center|XbaI
|align=center|XbaI
|align=center|PstI
|align=center|PstI
-
|align=center| FV
+
|align=center| BI
|align=center| 2057 pb
|align=center| 2057 pb
|align=center| 939 pb
|align=center| 939 pb
-
|align=center| 3000 pb
+
|align=center|  
-
|align=center| Not digested
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 3-5
+
|align=center|  
|-
|-
! rowspan="1" style="background: #ccccff;"|D122
! rowspan="1" style="background: #ccccff;"|D122
Line 500: Line 517:
|align=center|XbaI
|align=center|XbaI
|align=center|PstI
|align=center|PstI
-
|align=center| FV
+
|align=center| BI
|align=center| 2057 pb
|align=center| 2057 pb
|align=center| 900 pb
|align=center| 900 pb
-
|align=center| 2500 pb
+
|align=center|  
-
|align=center| Not digested
+
|align=center|  
-
|align=center| 6
+
|align=center|  
-
|align=center| 6-8
+
|align=center|  
|}
|}

Revision as of 14:10, 26 July 2008

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Contents

MiniPreps

  • The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.
Name Biobrick Description
MP116 J23100
MP117 J23107
MP118 B0015
MP119 I0500
MP120 B0030
MP121 E0422
MP122 E0840

Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2


Results of digestions : Electrophoresis

gel1 gel1 gel2 gel2 gel3 gel3 gel4 gel4


gel5 gel5 gel6 gel6 gel7 gel7 gel8 gel8


Name BioBrick Tube N° Enz 1 Enz 2 Obs Exp Size Matrix Exp Size BB Mea Size Matrix Mea Size BB Gel Band
D100 B0034 1 XbaI PstI BI 2057 pb 34 pb
2
3 EcoRI XbaI FV 2076 pb 15 pb
4 SpeI PstI BV 2077 pb 14 pb
5
D101 J23101 1 SpeI PstI BV 2100 pb 883 pb
2
D102 J23109 1 SpeI PstI BV 2100 pb 883 pb
D103 R0079 1 SpeI PstI BV 2222 pb 14 pb
2
D104 R0040 1 SpeI PstI BV 2119 pb 14 pb
2
D105 S03154 1 SpeI PstI BV 2750 pb 14 pb
2 XbaI PstI BI 2057 pb 707 pb
3
4 EcoRI SpeI FI 2056 pb 708 pb
D106 S03879 1 SpeI PstI BV 2768 pb 14 pb
2 XbaI PstI BI 2057 pb 725 pb
3
4 EcoRI SpeI FI 2756 pb 726 pb
D107 C0079 1 EcoRI SpeI FI 4402 pb 779 pb
2 XbaI PstI BI 4003 pb 778 pb
D108 C0179 1 EcoRI SpeI FI 4402 pb 746 pb
2 XbaI PstI BI 4403 pb 745 pb
D109 E0030 1 EcoRI SpeI FI 3166 pb 746 pb
2 XbaI PstI BI 3167 pb 745 pb
D110 E0040 1 EcoRI SpeI FI 2056 pb 743 pb
2 XbaI PstI BI 2057 pb 742 pb
D111 E1010 1 EcoRI SpeI FI 4402 pb 704 pb
2 XbaI PstI BI 4403 pb 703 pb
D116 J23100 1 SpeI PstI BV 2100 pb 883 pb
2
D117 J23107 1 SpeI PstI BV 2100 pb 883 pb
2
D118 B0015 1 EcoRI XbaI FV 3303 pb 15 pb
2
D119 I0500 1 SpeI PstI FV 5621 pb 14 pb
2
D120 B0030 1 XbaI PstI BI 2057 pb 37 pb
2
3 EcoRI XbaI FV 2079 pb 15 pb
4 SpeI PstI BV 2080 pb 14 pb
5
D121 E0422 1 XbaI PstI BI 2057 pb 939 pb
D122 E0840 1 XbaI PstI BI 2057 pb 900 pb
Retrieved from "http://2008.igem.org/Team:Paris/July_24"