Team:Paris/July 24

From 2008.igem.org

(Difference between revisions)

Revision as of 14:59, 26 July 2008

MiniPreps

The protocol of MiniPrep of Promega has been done on all the clones cultivated on the 23th.

Name	Biobrick	Description
MP116	J23100
MP117	J23107
MP118	B0015
MP119	I0500
MP120	B0030
MP121	E0422
MP122	E0840

Digestion

Digestion Mix

10µl of Miniprep (clone 1 or 2)
12.5µl of water
2.5µl of Buffer N°2
0.25µl of BSA 100x
1µl of enzyme 1
1µl of enzyme 2

Results of digestions : Electrophoresis

gel1 gel2 gel3 gel4

gel5 gel6 gel7 gel8

Name	BioBrick	Tube N°	Enz 1	Enz 2	Obs	Exp Size Matrix	Exp Size BB	Mea Size Matrix	Mea Size BB	Gel	Band
D100	B0034	1	XbaI	PstI	BI	2057 pb	34 pb	not digested	not digested	8	5-6
		2	XbaI	PstI	BI	2057 pb	34 pb	not digested	not digested	8	5-6
		3	EcoRI	XbaI	FV	2076 pb	15 pb	2100 pb	-	1	2
		4	SpeI	PstI	BV	2077 pb	14 pb	2000 pb	-	1	3-4
		5	SpeI	PstI	BV	2077 pb	14 pb	2000 pb	-	1	5-6
D101	J23101	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb	1	7-8
D101	J23101	2	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb	1	9-10
D102	J23109	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	900 pb	1 & 2	11-12 & 2-3
D103	R0079	1	SpeI	PstI	BV	2222 pb	14 pb	2500 pb	-	2	4-5
D103	R0079	2	SpeI	PstI	BV	2222 pb	14 pb	2500 pb	-	2	6-7
D104	R0040	1	SpeI	PstI	BV	2119 pb	14 pb	2500 pb	-	2	8-9
D104	R0040	2	SpeI	PstI	BV	2119 pb	14 pb	2500 pb	-	2	10-11
D105	S03154	1	SpeI	PstI	BV	2750 pb	14 pb	3000 pb	-	2 & 3	12 & 2
		2	XbaI	PstI	BI	2057 pb	707 pb	2000 pb	700 pb	3	3-4
		3	XbaI	PstI	BI	2057 pb	707 pb	2000 pb	700 pb	3	5-6
		4	EcoRI	SpeI	FI	2056 pb	708 pb	2000 pb	700 pb	3	7-8
D106	S03879	1	SpeI	PstI	BV	2768 pb	14 pb	3000 pb	-	3	9-10
		2	XbaI	PstI	BI	2057 pb	725 pb	2000 pb	700 pb	3	11-12
		3	XbaI	PstI	BI	2057 pb	725 pb	2000 pb	700 pb	4	2-3
		4	EcoRI	SpeI	FI	2756 pb	726 pb	2500 pb	700 pb	4	4-5
D107	C0079	1	EcoRI	SpeI	FI	4402 pb	779 pb	6000 pb	1000 pb	4	6-7
D107	C0079	2	XbaI	PstI	BI	4003 pb	778 pb	5000 pb	100 pb	6	9
D108	C0179	1	EcoRI	SpeI	FI	4402 pb	746 pb	2500 pb	1000 pb	4	8-9
D108	C0179	2	XbaI	PstI	BI	4403 pb	745 pb	2500 pb	1000 pb	6	10
D109	E0030	1	EcoRI	SpeI	FI	3166 pb	746 pb	4000 pb	1000 pb	4	10-11
D109	E0030	2	XbaI	PstI	BI	3167 pb	745 pb	3000 pb	1000 pb	6	11
D110	E0040	1	EcoRI	SpeI	FI	2056 pb	743 pb	2000 pb	800 pb	4 & 5	12 & 2
D110	E0040	2	XbaI	PstI	BI	2057 pb	742 pb	2500 pb	1000 pb	6	12
D111	E1010	1	EcoRI	SpeI	FI	4402 pb	704 pb	4000 pb	600 pb	5	3-4
D111	E1010	2	XbaI	PstI	BI	4403 pb	703 pb	4500 pb	700 pb	8	2-3
D116	J23100	1	SpeI	PstI	BV	2100 pb	883 pb	Not realised
D117	J23107	1	SpeI	PstI	BV	2100 pb	883 pb	2000 pb	800 pb	5	5-6
D118	B0015	1	EcoRI	XbaI	FV	3303 pb	15 pb	3000 pb	-	5	7-8
D119	I0500	1	SpeI	PstI	FV	5621 pb	14 pb	6000 - 3000 pb	-	5	9-10
D120	B0030	1	XbaI	PstI	BI	2057 pb	37 pb	not digested	not digested	7	2
		2	XbaI	PstI	BI	2057 pb	37 pb	not digested	not digested	7	5-6
		3	EcoRI	XbaI	FV	2079 pb	15 pb	1600 pb	-	6	2
		4	SpeI	PstI	BV	2080 pb	14 pb	3000 - 2000 pb	-	5	11
		5	SpeI	PstI	BV	2080 pb	14 pb	3000 - 2000 pb	-	5	12
D121	E0422	1	XbaI	PstI	FV	2057 pb	939 pb	3000 pb	Not digested	6	3-5
D122	E0840	1	XbaI	PstI	FV	2057 pb	900 pb	2500 pb	Not digested	6	6-8

@@ Line 10: / Line 10: @@
 |align="center"|'''Biobrick'''
 |align="center"|'''Description'''
-|-
-|align="center"|MP100
-|align="center"|[http://partsregistry.org/Part:BBa_B0034 B0034]
-|align="center"|Strongest RBS (Efficiency = 1)
-|-
-|align="center"|MP101
-|align="center"|[http://partsregistry.org/Part:BBa_J23101 J23101]
-|align="center"|Strongest Constitutive promoter
-|-
-|align="center"|MP102
-|align="center"|[http://partsregistry.org/Part:BBa_J23109 J23109]
-|align="center"|Weak Constitutive promoter
-|-
-|align="center"|MP103
-|align="center"|[http://partsregistry.org/Part:BBa_R0079 R0079]
-|align="center"|promoter pLas (lasr & PAI regulated)
-|-
-|align="center"|MP104
-|align="center"|[http://partsregistry.org/Part:BBa_R0040 R0040]
-|align="center"|tetR repressible promoter
-|-
-|align="center"|MP105
-|align="center"|[http://partsregistry.org/Part:BBa_S03154 S03154]
-|align="center"|B0034(rbs) ->LasI
-|-
-|align="center"|MP106
-|align="center"|[http://partsregistry.org/Part:BBa_S03879 S03879]
-|align="center"|B0034(rbs) -> TetR
-|-
-|align="center"|MP107
-|align="center"|[http://partsregistry.org/Part:BBa_C0079 C0079]
-|align="center"|lasR activator with LVA Tag
-|-
-|align="center"|MP108
-|align="center"|[http://partsregistry.org/Part:BBa_C0179 C0179]
-|align="center"|lasR activator
-|-
-|align="center"|MP109
-|align="center"|[http://partsregistry.org/Part:BBa_E0030 E0030]
-|align="center"|YFP
-|-
-|align="center"|MP110
-|align="center"|[http://partsregistry.org/Part:BBa_E0040 E0040]
-|align="center"|GFP
-|-
-|align="center"|MP111
-|align="center"|[http://partsregistry.org/Part:BBa_E1010 E1010]
-|align="center"|mRFP
 |-
 |align="center"|MP116
-|align="center"|[http://partsregistry.org/Part:BBa_J23100 J23100]
+|align="center"|J23100
-|align="center"|Strong constitutive promoter in J61002
+|align="center"|
 |-
 |align="center"|MP117
-|align="center"|[http://partsregistry.org/Part:BBa_J23107 J23107]
+|align="center"|J23107
-|align="center"|Medium constitutive promoter in J61002
+|align="center"|
 |-
 |align="center"|MP118
-|align="center"|[http://partsregistry.org/Part:BBa_B0015 B0015]
+|align="center"|B0015
-|align="center"|Double terminator
+|align="center"|
 |-
 |align="center"|MP119
-|align="center"|[http://partsregistry.org/Part:BBa_I0500 I0500]
+|align="center"|I0500
-|align="center"|AraC pBAD
+|align="center"|
 |-
 |align="center"|MP120
-|align="center"|[http://partsregistry.org/Part:BBa_B0030 B0030]
+|align="center"|B0030
-|align="center"|Strong RBS (Efficiency = 0,6)
+|align="center"|
 |-
 |align="center"|MP121
-|align="center"|[http://partsregistry.org/Part:BBa_E0422 E0422]
+|align="center"|E0422
-|align="center"|ECFP (RBS+LVA+Term)
+|align="center"|
 |-
-|align="center"| MP122
+|align="center"|MP122
-|align="center"|[http://partsregistry.org/Part:BBa_E0840 E0840]
+|align="center"|E0840
-|align="center"|gfp tri-part; strong rbs
+|align="center"|
-|
 |}
@@ Line 108: / Line 59: @@
-gel1 [[Image:| gel1|200px]]
+gel1 [[Image:Gel1.jpg| gel1|200px]]
-gel2 [[Image:| gel2|200px]]
+gel2 [[Image:Gel2.jpg| gel2|200px]]
-gel3 [[Image:| gel3|200px]]
+gel3 [[Image:Gel3.jpg| gel3|200px]]
-gel4 [[Image:| gel4|200px]]<br>
+gel4 [[Image:Gel4.jpg| gel4|200px]]<br>
-gel5 [[Image:‎| gel5|200px]]
+gel5 [[Image:Gel_5.jpg‎| gel5|200px]]
-gel6 [[Image:‎| gel6|200px]]
+gel6 [[Image:Gel_6.jpg‎| gel6|200px]]
-gel7 [[Image:| gel7|100px]]
+gel7 [[Image:Gel_7.jpg‎| gel7|100px]]
-gel8 [[Image:|100px]]
+gel8 [[Image:Gel_8.jpg‎| gel8|100px]]
@@ Line 143: / Line 94: @@
 ! rowspan="2"| 2057 pb
 ! rowspan="2"| 34 pb
-! rowspan="2"|
+! rowspan="2"| not digested
-! rowspan="2"|
+! rowspan="2"| not digested
-! rowspan="2"|
+! rowspan="2"| 8
-! rowspan="2"|
+! rowspan="2"| 5-6
 |-
 |align=center|2
@@ Line 156: / Line 107: @@
 |align=center| 2076 pb
 |align=center| 15 pb
-|align=center|
+|align=center| 2100 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| 1
-|align=center|
+|align=center| 2
 |-
 |align=center|4
@@ Line 167: / Line 118: @@
 ! rowspan="2"| 2077 pb
 ! rowspan="2"| 14 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center| 1
-|align=center|
+|align=center| 3-4
 |-
 |align=center|5
-|align=center|
+|align=center| 1
-|align=center|
+|align=center| 5-6
 |-
 ! rowspan="2" style="background: #ccccff;" | D101
@@ Line 184: / Line 135: @@
 ! rowspan="2"| 2100 pb
 ! rowspan="2"| 883 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="2"|
+! rowspan="2"| 900 pb
-|align=center|
+|align=center| 1
-|align=center|
+|align=center| 7-8
 |-
 |align=center|2
-|align=center|
+|align=center| 1
-|align=center|
+|align=center| 9-10
 |-
 ! rowspan="1" style="background: #ccccff;" | D102
@@ Line 201: / Line 152: @@
 ! rowspan="1"| 2100 pb
 ! rowspan="1"| 883 pb
-|align=center|
+|align=center| 2000 pb
-|align=center|
+|align=center| 900 pb
-|align=center|
+|align=center| 1 & 2
-|align=center|
+|align=center| 11-12 & 2-3
 |-
 ! rowspan="2" style="background: #ccccff;" | D103
@@ Line 214: / Line 165: @@
 ! rowspan="2"| 2222 pb
 ! rowspan="2"| 14 pb
-! rowspan="2"|
+! rowspan="2"| 2500 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center| 2
-|align=center|
+|align=center| 4-5
 |-
 |align=center|2
-|align=center|
+|align=center| 2
-|align=center|
+|align=center| 6-7
 |-
 ! rowspan="2" style="background: #ccccff;" | D104
@@ Line 231: / Line 182: @@
 ! rowspan="2"| 2119 pb
 ! rowspan="2"| 14 pb
-! rowspan="2"|
+! rowspan="2"| 2500 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center| 2
-|align=center|
+|align=center| 8-9
 |-
 |align=center|2
-|align=center|
+|align=center| 2
-|align=center|
+|align=center| 10-11
 |-
 ! rowspan="4" style="background: #ccccff;" | D105
@@ Line 248: / Line 199: @@
 ! rowspan="1"| 2750 pb
 ! rowspan="1"| 14 pb
-|align=center|
+|align=center| 3000 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| 2 & 3
-|align=center|
+|align=center| 12 & 2
 |-
 |align=center|2
@@ Line 259: / Line 210: @@
 ! rowspan="2"| 2057 pb
 ! rowspan="2"| 707 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="2"|
+! rowspan="2"| 700 pb
-|align=center|
+|align=center| 3
-|align=center|
+|align=center| 3-4
 |-
 |align=center|3
-|align=center|
+|align=center| 3
-|align=center|
+|align=center| 5-6
 |-
 |align=center|4
@@ Line 274: / Line 225: @@
 | align=center| 2056 pb
 | align=center| 708 pb
-|align=center|
+|align=center| 2000 pb
-|align=center|
+|align=center| 700 pb
-|align=center|
+|align=center| 3
-|align=center|
+|align=center| 7-8
 |-
 ! rowspan="4" style="background: #ccccff;" | D106
@@ Line 287: / Line 238: @@
 | align=center| 2768 pb
 | align=center|14 pb
-|align=center|
+|align=center| 3000 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| 3
-|align=center|
+|align=center| 9-10
 |-
 |align=center|2
@@ Line 298: / Line 249: @@
 ! rowspan="2"| 2057 pb
 ! rowspan="2"| 725 pb
-! rowspan="2"|
+! rowspan="2"| 2000 pb
-! rowspan="2"|
+! rowspan="2"| 700 pb
-|align=center|
+|align=center| 3
-|align=center|
+|align=center| 11-12
 |-
 |align=center|3
-|align=center|
+|align=center| 4
-|align=center|
+|align=center| 2-3
 |-
 |align=center|4
@@ Line 313: / Line 264: @@
 | align=center| 2756 pb
 | align=center| 726 pb
-|align=center|
+|align=center|  2500 pb
-|align=center|
+|align=center| 700 pb
-|align=center|
+|align=center| 4
-|align=center|
+|align=center| 4-5
 |-
 ! rowspan="2" style="background: #ccccff;" |D107
@@ Line 326: / Line 277: @@
 |align=center| 4402 pb
 |align=center| 779 pb
-|align=center|
+|align=center| 6000 pb
-|align=center|
+|align=center| 1000 pb
-|align=center|
+|align=center| 4
-|align=center|
+|align=center| 6-7
 |-
 |align=center| 2
@@ Line 337: / Line 288: @@
 |align=center| 4003 pb
 |align=center| 778 pb
-|align=center|
+|align=center| 5000 pb
-|align=center|
+|align=center| 100 pb
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 9
 |-
 ! rowspan="2" style="background: #ccccff;"|D108
@@ Line 350: / Line 301: @@
 |align=center| 4402 pb
 |align=center| 746 pb
-|align=center|
+|align=center| 2500 pb
-|align=center|
+|align=center| 1000 pb
-|align=center|
+|align=center| 4
-|align=center|
+|align=center| 8-9
 |-
 |align=center| 2
@@ Line 361: / Line 312: @@
 |align=center| 4403 pb
 |align=center| 745 pb
-|align=center|
+|align=center| 2500 pb
-|align=center|
+|align=center| 1000 pb
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 10
 |-
 ! rowspan="2" style="background: #ccccff;"|D109
@@ Line 374: / Line 325: @@
 |align=center| 3166 pb
 |align=center| 746 pb
-|align=center|
+|align=center| 4000 pb
-|align=center|
+|align=center| 1000 pb
-|align=center|
+|align=center| 4
-|align=center|
+|align=center| 10-11
 |-
 |align=center| 2
@@ Line 385: / Line 336: @@
 |align=center| 3167 pb
 |align=center| 745 pb
-|align=center|
+|align=center| 3000 pb
-|align=center|
+|align=center| 1000 pb
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 11
 |-
 ! rowspan="2" style="background: #ccccff;"|D110
@@ Line 398: / Line 349: @@
 |align=center| 2056 pb
 |align=center| 743 pb
-|align=center|
+|align=center| 2000 pb
-|align=center|
+|align=center| 800 pb
-|align=center|
+|align=center| 4 & 5
-|align=center|
+|align=center| 12 & 2
 |-
 |align=center| 2
@@ Line 409: / Line 360: @@
 |align=center| 2057 pb
 |align=center| 742 pb
-|align=center|
+|align=center| 2500 pb
-|align=center|
+|align=center| 1000 pb
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 12
 |-
 ! rowspan="2" style="background: #ccccff;"|D111
@@ Line 422: / Line 373: @@
 |align=center| 4402 pb
 |align=center| 704 pb
-|align=center|
+|align=center| 4000 pb
-|align=center|
+|align=center| 600 pb
-|align=center|
+|align=center| 5
-|align=center|
+|align=center| 3-4
 |-
 |align=center| 2
@@ Line 433: / Line 384: @@
 |align=center| 4403 pb
 |align=center| 703 pb
-|align=center|
+|align=center| 4500 pb
-|align=center|
+|align=center| 700 pb
-|align=center|
+|align=center| 8
-|align=center|
+|align=center| 2-3
 |-
-! rowspan="2" style="background: #ccccff;"|D116
+! rowspan="1" style="background: #ccccff;"|D116
-! rowspan="2" style="background: #ccccff;"|J23100
+! rowspan="1" style="background: #ccccff;"|J23100
 |align=center|1
-! rowspan="2"| SpeI
+! rowspan="1"| SpeI
-! rowspan="2"| PstI
+! rowspan="1"| PstI
-! rowspan="2"| BV
+! rowspan="1"| BV
-! rowspan="2"| 2100 pb
+! rowspan="1"| 2100 pb
-! rowspan="2"| 883 pb
+! rowspan="1"| 883 pb
-! colspan="4"|
+! colspan="4"| Not realised
 |align=center|
 |-
-|align=center|2
+! rowspan="1" style="background: #ccccff;"|D117
-|
+! rowspan="1" style="background: #ccccff;"|J23107
-|
-|-
-! rowspan="2" style="background: #ccccff;"|D117
-! rowspan="2" style="background: #ccccff;"|J23107
 |align=center|1
-! rowspan="2"| SpeI
+! rowspan="1"| SpeI
-! rowspan="2"| PstI
+! rowspan="1"| PstI
-! rowspan="2"| BV
+! rowspan="1"| BV
-! rowspan="2"| 2100 pb
+! rowspan="1"| 2100 pb
-! rowspan="2"| 883 pb
+! rowspan="1"| 883 pb
-|align=center|
+|align=center| 2000 pb
-|align=center|
+|align=center| 800 pb
-|align=center|
+|align=center| 5
-|align=center|
+|align=center| 5-6
-|align=center|
 |-
-|align=center|2
+! rowspan="1" style="background: #ccccff;"|D118
-|
+! rowspan="1" style="background: #ccccff;"|B0015
-|-
-! rowspan="2" style="background: #ccccff;"|D118
-! rowspan="2" style="background: #ccccff;"|B0015
 |align=center|1
 |align=center|EcoRI
 |align=center|XbaI
-! rowspan="2"| FV
+! rowspan="1"| FV
-! rowspan="2"| 3303 pb
+! rowspan="1"| 3303 pb
-! rowspan="2"| 15 pb
+! rowspan="1"| 15 pb
-|align=center|
+|align=center| 3000 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| 5
-|align=center|
+|align=center| 7-8
-|align=center|
-|-
-|align=center|2
-|
 |-
-! rowspan="2" style="background: #ccccff;"|D119
+! rowspan="1" style="background: #ccccff;"|D119
-! rowspan="2" style="background: #ccccff;"|I0500
+! rowspan="1" style="background: #ccccff;"|I0500
 |align=center|1
 |align=center|SpeI
 |align=center|PstI
-! rowspan="2"| FV
+! rowspan="1"| FV
-! rowspan="2"| 5621 pb
+! rowspan="1"| 5621 pb
-! rowspan="2"| 14 pb
+! rowspan="1"| 14 pb
-! rowspan="2"|
+|align=center| 6000 - 3000 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| 5
-|align=center|
+|align=center| 9-10
-|align=center|
-|-
-|align=center|2
-|
-|
 |-
 ! rowspan="5" style="background: #ccccff;" | D120
@@ Line 513: / Line 447: @@
 ! rowspan="2"| 2057 pb
 ! rowspan="2"| 37 pb
-! rowspan="2"|
+! rowspan="2"| not digested
-! rowspan="2"|
+! rowspan="2"| not digested
-|align=center|
+|align=center| 7
-|align=center|
+|align=center| 2
 |-
 |align=center|2
-|align=center|
+|align=center| 7
-|align=center|
+|align=center| 5-6
 |-
 |align=center|3
@@ Line 528: / Line 462: @@
 |align=center| 2079 pb
 |align=center| 15 pb
-|align=center|
+|align=center| 1600 pb
-|align=center|
+|align=center| -
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 2
 |-
 |align=center|4
@@ Line 539: / Line 473: @@
 ! rowspan="2"| 2080 pb
 ! rowspan="2"| 14 pb
-! rowspan="2"|
+! rowspan="2"| 3000 - 2000 pb
-! rowspan="2"|
+! rowspan="2"| -
-|align=center|
+|align=center| 5
-|align=center|
+|align=center| 11
 |-
 |align=center|5
-|align=center|
+|align=center| 5
-|align=center|
+|align=center| 12
 |-
 ! rowspan="1" style="background: #ccccff;"|D121
@@ Line 553: / Line 487: @@
 |align=center|XbaI
 |align=center|PstI
-|align=center| BI
+|align=center| FV
 |align=center| 2057 pb
 |align=center| 939 pb
-|align=center|
+|align=center| 3000 pb
-|align=center|
+|align=center| Not digested
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 3-5
 |-
 ! rowspan="1" style="background: #ccccff;"|D122
@@ Line 566: / Line 500: @@
 |align=center|XbaI
 |align=center|PstI
-|align=center| BI
+|align=center| FV
 |align=center| 2057 pb
 |align=center| 900 pb
-|align=center|
+|align=center| 2500 pb
-|align=center|
+|align=center| Not digested
-|align=center|
+|align=center| 6
-|align=center|
+|align=center| 6-8
 |}

Team:Paris/July 24

From 2008.igem.org

Revision as of 14:59, 26 July 2008

Contents

MiniPreps

Digestion

Digestion Mix

Results of digestions : Electrophoresis