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Team:Valencia/Notebook/July
From 2008.igem.org
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=== July 7th === | === July 7th === | ||
| - | - We prepared some inoculum. | + | - We prepared some inoculum for our experiment the following day. |
=== July 8th === | === July 8th === | ||
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===July 9th=== | ===July 9th=== | ||
| - | - We characterized culture growth in our calorimeters | + | - We characterized culture growth in our calorimeters with just one strain. |
| - | === | + | ===July 10th=== |
- Trying to see if there is basal rate of UCP1... | - Trying to see if there is basal rate of UCP1... | ||
| - | === | + | ===July 11th=== |
- It looks like our ''Saccharomyces cerevisiae'' strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure. | - It looks like our ''Saccharomyces cerevisiae'' strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure. | ||
| - | === | + | ===July 12th=== |
- We obtained the same results. | - We obtained the same results. | ||
| - | === | + | ===July 14th=== |
- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008. | - The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008. | ||
| - | === | + | ===July 15th=== |
- We prepared inoculum. | - We prepared inoculum. | ||
| - | === | + | ===July 16th=== |
- Trying to find out which is the best moment for activating UCP1... | - Trying to find out which is the best moment for activating UCP1... | ||
| - | === | + | ===July 17th=== |
| - | - Our lab electricity went | + | - Our lab electricity went off during the night. How many experiments will have it ruined? |
| - | === | + | ===July 18th=== |
| - | + | - We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose. | |
| - | + | ||
| - | + | - We carried out the pilot experiment. We see weird oscillations. | |
| - | - | + | |
| - | === | + | ===July 20th=== |
| + | - We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking. | ||
| + | |||
| + | ===July 21st=== | ||
- We carried out the pilot experiment again. Weird results, we need to repeat it. | - We carried out the pilot experiment again. Weird results, we need to repeat it. | ||
| - | === | + | ===July 22nd=== |
- We repaired our calorimeters. They were the reason for yesterday weird results. | - We repaired our calorimeters. They were the reason for yesterday weird results. | ||
| - | === | + | ===July 23rd=== |
- We repeated the pilot experiment. | - We repeated the pilot experiment. | ||
| - | === | + | ===July 24th=== |
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste... | - Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste... | ||
| - | === | + | ===July 25th=== |
- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't. | - Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't. | ||
| - | === | + | ===July 26th=== |
- We obtain good results. | - We obtain good results. | ||
| - | === | + | ===July 28th=== |
| - | + | - One of our calorimeters wasn't working. We repaired it and calibrated once again. | |
| - | === | + | |
| - | + | ===July 29th=== | |
| + | - Our cultures haven't grown enough for the experiment. Temperature and medium was all right, we'll have to wait... | ||
Revision as of 13:51, 4 August 2008
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July 1st
- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial.
- We calibrated our calorimeters with hot water.
July 2nd
- We prepared our first SD medium.
- We calibrated our calorimeters with hot water.
July 4th
- We prepared some plates for inoculum.
July 7th
- We prepared some inoculum for our experiment the following day.
July 8th
- We carried out the pilot experiment.
July 9th
- We characterized culture growth in our calorimeters with just one strain.
July 10th
- Trying to see if there is basal rate of UCP1...
July 11th
- It looks like our Saccharomyces cerevisiae strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.
July 12th
- We obtained the same results.
July 14th
- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.
July 15th
- We prepared inoculum.
July 16th
- Trying to find out which is the best moment for activating UCP1...
July 17th
- Our lab electricity went off during the night. How many experiments will have it ruined?
July 18th
- We prepared lots of plates with our SD medium...Unfortunately, we forgot to add glucose.
- We carried out the pilot experiment. We see weird oscillations.
July 20th
- We stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.
July 21st
- We carried out the pilot experiment again. Weird results, we need to repeat it.
July 22nd
- We repaired our calorimeters. They were the reason for yesterday weird results.
July 23rd
- We repeated the pilot experiment.
July 24th
- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...
July 25th
- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.
July 26th
- We obtain good results.
July 28th
- One of our calorimeters wasn't working. We repaired it and calibrated once again.
