Team:BCCS-Bristol/Calendar-Notebook/14 July 2008

From 2008.igem.org

(Difference between revisions)
Line 21: Line 21:
Today, the plates were photographed and measured. There was some significant movement more towards the aspartate (chemoattractant), but the difference should be more visible. Thus, various conditions should be tested during the next experiments.  
Today, the plates were photographed and measured. There was some significant movement more towards the aspartate (chemoattractant), but the difference should be more visible. Thus, various conditions should be tested during the next experiments.  
-
==Miscellaneous==
+
==Comparision of motility between ''E. coli'' MG1655 and MC1000==
-
The ordered ''E. coli'' MC1000 strain arrived today. It was decided to test the motility of this compared to MG1655 on overnight plates.
+
The agar plates were inoculated. Details on [[Team:BCCS-Bristol/Calendar-Notebook/15 July 2008 | 15 July 2008]].

Revision as of 09:29, 6 August 2008

Swimming agar assay with L-aspartic acid

This time, only 0.3 % agar plates were used. Two wells were made using the thick end of a 1000 µl pipette tip. In the left hole some aspartate solution was put and on the right some water (control). To half of the plates the solutions were added in the morning to allow chemotactic gradient to set up, to the second half the solutions were added immediately before the inoculation in the afternoon (arond 5 hours later). The E. coli MG1655 strain was inoculated three times on a line in the centre between the two holes and incubated overnight at the different temperatures: 25oC, 30oC and 37oC.

Today, the plates were photographed and measured. There was some significant movement more towards the aspartate (chemoattractant), but the difference should be more visible. Thus, various conditions should be tested during the next experiments.

Comparision of motility between E. coli MG1655 and MC1000

The agar plates were inoculated. Details on 15 July 2008.