Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(Parts stored in the fridge in the lab (4°C))
(Parts stored in the fridge in the lab (4°C))
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|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 30||104.0||22||July 29
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 30||104.0||22||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||170.8||45||July 29
+
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 35||170.8||45||July 29
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||159.0||46||July 29
|[http://partsregistry.org/Part:BBa_F1610 F1610]||pSB1AK3||RBS + LuxI + Terminator||+/- 40||159.0||46||July 29

Revision as of 10:43, 7 August 2008

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Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 4050.41July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 3054.32July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.85July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.16July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.07July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 25177.18July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.79July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.010July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.311July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.312July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 45303.113July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30213.614July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 48311.031July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40214.047July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40209.548July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.015July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.916July 24
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS (efficiency 0.3)+/- 10114.818July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4084.219July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4086.420July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.121July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40146.449July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40203.150July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 35117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 48129.128July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 40160.725July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 46308.329July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 46375.126July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4840430July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.227July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 30104.022July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 35170.845July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 40159.046July 29
[http://partsregistry.org/Part:BBa_I712022 I712022]BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
[http://partsregistry.org/Part:BBa_R0062 R0062]pSB1A2LuxR regulated promoter +/- 40104.137July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter+/- 4564.043July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter +/- 4069.844July 29
[http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 48119.138August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48131.039August 06
[http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for TetR generator+/- 48133.240August 06
[http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Reset+/- 48118.541August 06
[http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Cell Death+/- 4641.042August 06


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.61July 24
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert677257.12July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.83July 24
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert779229.54July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52214.25July 29
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125286.16July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert62233.47July 29
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090212.98July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090451.39July 31
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3308255.122August 07
[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR IXba IVector2077204.010July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33033111.711July 31
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33032513.420August 07
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72208.512July 30
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72207.513July 30
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert7741824.714August 01
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert806158.215August 01
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] EcoR ISpe IPlasmid20791718.216August 01
[http://partsregistry.org/Part:BBa_P1010 P1010] EcoR ISpe IInsert682104.917August 01
[http://partsregistry.org/Part:BBa_K145015 K145015] (GFP-LVA) EcoR ISpe IInsert625136.318August 05
[http://partsregistry.org/Part:BBa_C0012 C0012] EcoR ISpe IInsert1145255.619August 05
[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR IXba IVector34982513.221August 07

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer108499020 µM1August 01
Primer108599020 µM2August 01
dNTP mixATP, GTP, TTP, CTP99010 mM3August 01
Primer1084500100 µM4August 01
Primer1085500100 µM5August 01