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Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
(→Ligations: step 1) |
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|width=150| '''Confirmed''' | |width=150| '''Confirmed''' | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | | | + | | R0053+P0152 ||-||-||-||-||- |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | | | + | | R1052+P0353 ||-||-||-||-||- |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | | | + | | R0084+B0033 ||-||-||-||-||- |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | | | + | | C0056+B0015 ||-||-||-||-||- |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
| - | | ? ||-||-||-||-||- | + | | antisense? ||-||-||-||-||- |
|- style="background: #003E81;color:white;width=100%" | |- style="background: #003E81;color:white;width=100%" | ||
! colspan="6" style="background: #003E81" | Filter | ! colspan="6" style="background: #003E81" | Filter | ||
Revision as of 07:32, 12 August 2008
dock/undock dropdown
Parts stored in the fridge in the lab (4°C)
| Plasmids: MiniPrep Products | ||||||
|---|---|---|---|---|---|---|
| Part | Plasmid | Description | µl left | ng/µl | Box | Date |
| [http://partsregistry.org/Part:BBa_M30109 M30109] | pSB1AC3 | Light-sensor | +/- 40 | 50.4 | 1 | July 23 |
| [http://partsregistry.org/Part:BBa_M30109 M30109] | pSB1AC3 | Light-sensor | +/- 30 | 54.3 | 2 | July 24 |
| [http://partsregistry.org/Part:BBa_J23100 J23100] | BBa_J61002 (Ap) | Constitutive promoter | +/- 60 | 345.0 | 3 | July 24 |
| [http://partsregistry.org/Part:BBa_J23100 J23100] | BBa_J61002 (Ap) | Constitutive promoter | +/- 30 | 176.1 | 4 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 5 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 6 | July 24 |
| [http://partsregistry.org/Part:BBa_B0034 B0034] | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 7 | July 29 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | pSB1A2 | TetR repressor | +/- 25 | 177.1 | 8 | July 24 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 9 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 63.0 | 10 | July 23 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 10 | 112.3 | 11 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 20 | 130.3 | 12 | July 24 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 45 | 303.1 | 13 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 30 | 213.6 | 14 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 48 | 311.0 | 31 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 214.0 | 47 | July 29 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Terminator | +/- 40 | 209.5 | 48 | July 29 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 15 | July 23 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | pSB1A2 | OmpF promoter | +/- 30 | 60.9 | 16 | July 24 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS (efficiency 0.3) | +/- 10 | 114.8 | 18 | July 24 |
| [http://partsregistry.org/Part:BBa_E0022 E0022] | pSB1A2 | eCFP | +/- 40 | 84.2 | 19 | July 23 |
| [http://partsregistry.org/Part:BBa_E0022 E0022] | pSB1A2 | eCFP | +/- 40 | 86.4 | 20 | July 24 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI | +/- 30 | 133.1 | 21 | July 29 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI repressor | +/- 40 | 146.4 | 49 | July 29 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | pSB1A2 | LacI repressor | +/- 40 | 203.1 | 50 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | 3d lock | +/- 48 | 129.1 | 28 | July 29 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | 3d key+ terminator | +/- 40 | 160.7 | 25 | July 29 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | BBa_J23006 (Ap) | 3d key+ terminator | +/- 46 | 308.3 | 29 | July 29 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 46 | 375.1 | 26 | July 29 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 404 | 30 | July 29 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 27 | July 29 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | RBS + LuxI + Terminator | +/- 30 | 104.0 | 22 | July 29 |
| [http://partsregistry.org/Part:BBa_F1610 F1610] | pSB1AK3 | RBS + LuxI + Terminator | +/- 35 | 170.8 | 45 | July 29 |
| [http://partsregistry.org/Part:BBa_I712022 I712022] | BBa_J52017 (Km, Ap) | T7 polymerase | +/- 40 | 394.1 | 35 | July 31 |
| [http://partsregistry.org/Part:BBa_R0062 R0062] | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 37 | July 31 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1A2 | LacI regulated promoter | +/- 45 | 64.0 | 43 | July 29 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | pSB1A2 | LacI regulated promoter | +/- 40 | 69.8 | 44 | July 29 |
| [http://partsregistry.org/Part:BBa_I12006 I12006] | pSB2K3 | 434 cI repressible promoter | +/- 48 | 25.8 | 51 | August 08 |
| [http://partsregistry.org/Part:BBa_I12006 I12006] | pSB2K3 | 434 cI repressible promoter | +/- 48 | 52.7 | 52 | August 08 |
| [http://partsregistry.org/Part:BBa_J23116 J23116] | BBa_J61002 (Ap) | Constitutive promoter | +/- 48 | 328.8 | 53 | August 08 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | pSB1A2 | lactonase production | +/- 48 | 84.2 | 60 | August 11 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | pSB1A2 | LuxR repressor | +/- 48 | 76.2 | 61 | August 11 |
| [http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | RBS(medium) | +/- 48 | 51.0 | 62 | August 11 |
| [http://partsregistry.org/Part:BBa_R0040 R0040] | pSB1A2 | tetR repressible promoter | +/- 48 | 37.9 | 63 | August 11 |
| [http://partsregistry.org/Part:BBa_P1010 P1010]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Cell Death | +/- 48 | 119.1 | 38 | August 06 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for TetR generator | +/- 48 | 131.0 | 39 | August 06 |
| [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for TetR generator | +/- 48 | 133.2 | 40 | August 06 |
| [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Reset | +/- 48 | 118.5 | 41 | August 06 |
| [http://partsregistry.org/Part:BBa_J23100 J23100]+[http://partsregistry.org/Part:BBa_B0032 B0032] | pSB1A2 | Ligation for Cell Death | +/- 46 | 41.0 | 42 | August 06 |
| [http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Cell Death | +/- 46 | 124.8 | 54 | August 08 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2] | pSB1A2 | GFP with LVA tag | +/- 46 | 152.7 | 55 | August 08 |
| [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1A2 | Ligation for Output | +/- 46 | 17.5 | 56 | August 08 |
| [http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 89.9 | 57 | August 08 |
| [http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 94.4 | 58 | August 08 |
| [http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] | pSB1AK3 | Ligation for Output | +/- 46 | 151.8 | 59 | August 08 |
| Restriction Digests | ||||||||
|---|---|---|---|---|---|---|---|---|
| Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
| [http://partsregistry.org/Part:BBa_J23100 J23100] | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 1 | July 24 |
| [http://partsregistry.org/Part:BBa_C0040 C0040] | EcoR I | Spe I | Insert | 677 | 25 | 7.1 | 2 | July 24 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 3 | July 24 |
| [http://partsregistry.org/Part:BBa_E0022 E0022] | EcoR I | Spe I | Insert | 779 | 22 | 9.5 | 4 | July 24 |
| [http://partsregistry.org/Part:BBa_J23109 J23109] | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 5 | July 29 |
| [http://partsregistry.org/Part:BBa_R0084 R0084] | EcoR I | Spe I | Insert | 125 | 25 | 6.1 | 6 | July 29 |
| [http://partsregistry.org/Part:BBa_I712074 I712074] | EcoR I | Spe I | Insert | 62 | 23 | 3.4 | 7 | July 29 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 8 | July 30 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 9 | July 31 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3308 | 25 | 5.1 | 22 | August 07 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 31 | 11.7 | 11 | July 31 |
| [http://partsregistry.org/Part:BBa_B0015 B0015] | EcoR I | Xba I | Vector | 3303 | 21 | 13.4 | 20 | August 07 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 12 | July 30 |
| [http://partsregistry.org/Part:BBa_R0011 R0011] | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 13 | July 30 |
| [http://partsregistry.org/Part:BBa_C0062 C0062] | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 14 | August 01 |
| [http://partsregistry.org/Part:BBa_C0060 C0060] | EcoR I | Spe I | Insert | 806 | 15 | 8.2 | 15 | August 01 |
| [http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] | EcoR I | Spe I | Plasmid | 2079 | 17 | 18.2 | 16 | August 01 |
| [http://partsregistry.org/Part:BBa_P1010 P1010] | EcoR I | Spe I | Insert | 682 | 10 | 4.9 | 17 | August 01 |
| [http://partsregistry.org/Part:BBa_K145015 K145015] (GFP-LVA) | EcoR I | Spe I | Insert | 625 | 13 | 6.3 | 18 | August 05 |
| [http://partsregistry.org/Part:BBa_C0012 C0012] | EcoR I | Spe I | Insert | 1145 | 12 | 5.6 | 19 | August 05 |
| [http://partsregistry.org/Part:BBa_J23022 J23022] | EcoR I | Xba I | Vector | 3498 | 20 | 15.2 | 21 | August 07 |
| [http://partsregistry.org/Part:BBa_R0062 R0062] | EcoR I | Spe I | Insert | 55 | 27 | 7.8 | 23 | August 07 |
| [http://partsregistry.org/Part:BBa_J23032 J23032] | EcoR I | Xba I | Vector | 3305 | +/-25 | 5.1 | 22 | August 07 |
Things stored in freezer in lab (-20°C)
| Miscellaneous | |||||
|---|---|---|---|---|---|
| Description | Name | µl left | Concentration | Box | Date |
| Primer GFP-LVA | 1084 | 90 | 20 µM | 1 | August 01 |
| Primer GFP-LVA | 1085 | 90 | 20 µM | 2 | August 01 |
| dNTP mix | ATP, GTP, TTP, CTP | 70 | 10 mM | 3 | August 01 |
| Primer GFP-LVA | 1084 | 500 | 100 µM | 4 | August 01 |
| Primer GFP-LVA | 1085 | 500 | 100 µM | 5 | August 01 |
| Primer Test | 1074 | 800 | 100 µM | 6 | August 07 |
| Primer Test | 1075 | 780 | 100 µM | 7 | August 07 |
| Primer T7-UmuD | 1076 | 560 | 100 µM | 8 | August 07 |
| Primer T7-UmuD | 1077 | 477 | 100 µM | 9 | August 07 |
| Primer T7-UmuD | 1078 | 467 | 100 µM | 10 | August 07 |
| Primer T7-UmuD | 1079 | 45 | 100 µM | 11 | August 07 |
| Primer Transduction | 1097 | 627 | 100 µM | 12 | August 07 |
| Primer Transduction | 1098 | 710 | 100 µM | 13 | August 07 |
| Primer Transduction | 1099 | 608 | 100 µM | 14 | August 07 |
| Primer Anti-sense LuxI | 1100 | 522 | 100 µM | 15 | August 07 |
| Primer Anti-sense LuxI | 1101 | 237 | 100 µM | 16 | August 07 |
| Primer Test | 1074 | 100 | 20 µM | 17 | August 07 |
| Primer Test | 1075 | 100 | 20 µM | 18 | August 07 |
| Primer T7-UmuD | 1076 | 100 | 20 µM | 19 | August 07 |
| Primer T7-UmuD | 1077 | 100 | 20 µM | 20 | August 07 |
| Primer T7-UmuD | 1078 | 100 | 20 µM | 21 | August 07 |
| Primer T7-UmuD | 1079 | 100 | 20 µM | 22 | August 07 |
| Primer Transduction | 1097 | 100 | 20 µM | 23 | August 07 |
| Primer Transduction | 1098 | 100 | 20 µM | 24 | August 07 |
| Primer Transduction | 1099 | 100 | 20 µM | 25 | August 07 |
| Primer Anti-sense LuxI | 1100 | 100 | 20 µM | 26 | August 07 |
| Primer Anti-sense LuxI | 1101 | 100 | 20 µM | 27 | August 07 |
| dNTP mix | ATP, GTP, TTP, CTP | 90 | 2 mM | 28 | August 07 |
Ligations: step 1
| Inputs | |||||
|---|---|---|---|---|---|
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| J23116+B0032 | - | - | - | - | - |
| C0040+B0015 | ok | ok | - | - | - |
| R0040+E0240 | - | - | - | - | - |
| Output | |||||
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| R0084+B0032 | ok | ok | ok | - | - |
| E0022+B0015 | ok | ok | ok | ok | ok |
| K145015+B0015 | pcr | ok | ok | ok | - |
| E0040+B0015 | - | - | - | - | - |
| Memory | |||||
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| R0053+P0152 | - | - | - | - | - |
| R1052+P0353 | - | - | - | - | - |
| R0084+B0033 | - | - | - | - | - |
| C0056+B0015 | - | - | - | - | - |
| antisense? | - | - | - | - | - |
| Filter | |||||
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| R0084+J23022 | ok | ok | ok | - | - |
| J230109+J23032 | ok | ok | ok | ok | - |
| K145014+B0015 | - | - | - | - | - |
| K145001+B0015 | - | - | - | - | - |
| I7122074+J23032 | ok | ok | ok | ok | - |
| IK0145015+B0015 | pcr | ok | ok | ok | - |
| E0040+B0015 | - | - | - | - | - |
| Inverter | |||||
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| I712074+J23032 | ok | ok | ok | ok | - |
| C0012+B0015 | ok | ok | ok | - | - |
| R0011+F1610 | ok | - | - | - | - |
| R0011+E0240 | - | - | - | - | - |
| Reset | |||||
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| I712074+J023022 | ok | ok | ok | ok | - |
| C0012+B0015 | - | ok | ok | ok | - |
| Cell Death | |||||
| Name | Miniprep | Digest | Ligation | Colonies | Confirmed |
| J23100+B0032 | ok | ok | ok | ok | - |
| C0062+B0015 | - | ok | ok | ok | ok |
| R0062+B0032 | ok | ok | ok | - | - |
| P1010+B0015 | - | ok | ok | ok | - |
| R0062+E0240 | - | - | - | - | - |
