Team:Valencia/Notebook/August
From 2008.igem.org
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===August 7th=== | ===August 7th=== | ||
+ | -We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment: <br> | ||
+ | [[Team:Valencia/Notebook/Protocols#SP liquid medium | SP medium]]. <br> | ||
+ | Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating. | ||
+ | Strain UCP+ just with galactose induction. | ||
<html> | <html> |
Revision as of 19:33, 12 August 2008
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August 1st
- We carried out the experiment with Medium YPKAc. We were just characterizing yeast growth in this medium. In this medium containing acetate, yeast is supposed to only breathe rather than fermenting.
August 5th
- Experiment carried out:
SP medium.
Four strains.
No galactose activation.
August 6th
-Trying to find out the best moment for galactose activation....
SP medium.
Galactose activation at two, three and four hours.
D.O. measured at each time.
August 7th
-We added palmitic acid to see if it had any effect, especially in UCP+ strain. Experiment:
SP medium.
Strains UCP-, 76Δ and UCP+ with galactose induction and palmitic acid at 3 hours after inoculating.
Strain UCP+ just with galactose induction.