Team:Paris/August 2
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AnaJimenez (Talk | contribs) (New page: {| border="1" |align="center"|'''Ref. Book''' |align="center"|'''Biobricks 2007''' |align="center"|'''Antibio''' |align="center"|'''Results''' |- |align="center"| - |align="center"|posit...) |
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+ | {{Paris/Calendar_Links|August 1|August 3}} | ||
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+ | == Transformation of 1st August == | ||
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+ | === Results of the transformation === | ||
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{| border="1" | {| border="1" | ||
- | |align="center"|''' | + | |align="center"|'''Ligation name''' |
- | |align="center"|''' | + | |align="center"|'''Description''' |
|align="center"|'''Antibio''' | |align="center"|'''Antibio''' | ||
- | |align="center"|''' | + | |align="center"|'''Nb colonies observed''' |
+ | |align="center"|'''Fluorescence''' | ||
+ | |align="center"|'''Comments''' | ||
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- | |align="center"| | + | |align="center"|puc19 |
- | |align="center"|positive control | + | |align="center"|positive control |
- | |align="center"| | + | |align="center"|Amp |
- | |align="center"| | + | |align="center"| |
+ | |align="center"|- | ||
+ | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| - | + | |align="center"|L100 |
- | |align="center"| | + | |align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI) |
- | |align="center"| | + | |align="center"|Amp |
- | |align="center"| | + | |align="center"|608 |
+ | |align="center"|No | ||
+ | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L101 |
- | |align="center"| | + | |align="center"|rbs TetR - GFP tripart<br>D110 (BV) - D131 (BI) |
- | |align="center"| | + | |align="center"|Amp |
- | |align="center"| | + | |align="center"|> 4000 |
+ | |align="center"|GFP positive | ||
+ | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L113 |
- | |align="center"| | + | |align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI) |
- | |align="center"| | + | |align="center"|Kana |
- | |align="center"| | + | |align="center"|9 |
+ | |align="center"| | ||
+ | |align="center"| | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L114 |
- | |align="center"| | + | |align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI) |
- | |align="center"| | + | |align="center"|Kana |
- | |align="center"| | + | |align="center"|9 |
+ | |align="center"| | ||
+ | |align="center"| | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L120 |
- | |align="center"| | + | |align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI) |
- | |align="center"| | + | |align="center"|Amp |
- | |align="center"| | + | |align="center"|74 |
+ | |align="center"| | ||
+ | |align="center"| | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L122 |
- | |align="center"| | + | |align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI) |
- | |align="center"| | + | |align="center"|Amp |
- | |align="center"| | + | |align="center"|4 |
+ | |align="center"|No | ||
+ | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L123 |
- | |align="center"| | + | |align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI) |
- | |align="center"| | + | |align="center"|Amp |
- | |align="center"| | + | |align="center"|> 4000 |
+ | |align="center"|No | ||
+ | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L126 |
- | |align="center"| | + | |align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI) |
- | + | |align="center"|Amp | |
- | + | |align="center"|944 | |
- | + | |align="center"|No | |
- | + | |align="center"|OK | |
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|} | |} | ||
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+ | [[Image:transfo.jpg|frame|center|L101]] | ||
+ | ==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |
Latest revision as of 17:09, 13 August 2008
Transformation of 1st AugustResults of the transformation
==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |