Team:Paris/August 2
From 2008.igem.org
(Difference between revisions)
AnaJimenez (Talk | contribs) (→Results of the transformation) |
(→Results of the transformation) |
||
(29 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | {{Paris/Calendar_Links|August 1 |August | + | {{Paris/Calendar_Links|August 1|August 3}} |
+ | |||
+ | |||
+ | |||
+ | == Transformation of 1st August == | ||
=== Results of the transformation === | === Results of the transformation === | ||
Line 12: | Line 16: | ||
|align="center"|'''Comments''' | |align="center"|'''Comments''' | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|puc19 |
- | |align="center"| | + | |align="center"|positive control |
- | + | |align="center"|Amp | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | |align="center"| | + | |
|align="center"| | |align="center"| | ||
+ | |align="center"|- | ||
|align="center"|OK | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L100 |
- | |align="center"| | + | |align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI) |
- | + | |align="center"|Amp | |
- | <br> | + | |align="center"|608 |
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |
- | |align="center"| | + | |
|align="center"|OK | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L101 |
- | |align="center"| | + | |align="center"|rbs TetR - GFP tripart<br>D110 (BV) - D131 (BI) |
- | + | |align="center"|Amp | |
- | <br> | + | |align="center"|> 4000 |
- | |align="center"| | + | |align="center"|GFP positive |
- | |align="center"| | + | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | |align="center"| | + | |
|align="center"|OK | |align="center"|OK | ||
|- | |- | ||
- | |align="center"| | + | |align="center"|L113 |
+ | |align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI) | ||
+ | |align="center"|Kana | ||
+ | |align="center"|9 | ||
|align="center"| | |align="center"| | ||
- | |||
- | |||
|align="center"| | |align="center"| | ||
- | |||
- | |||
- | |||
|- | |- | ||
- | |align="center"| | + | |align="center"|L114 |
+ | |align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI) | ||
+ | |align="center"|Kana | ||
+ | |align="center"|9 | ||
|align="center"| | |align="center"| | ||
- | |||
- | |||
|align="center"| | |align="center"| | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
|- | |- | ||
|align="center"|L120 | |align="center"|L120 | ||
+ | |align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI) | ||
+ | |align="center"|Amp | ||
+ | |align="center"|74 | ||
|align="center"| | |align="center"| | ||
- | |||
- | |||
|align="center"| | |align="center"| | ||
- | |||
- | |||
- | |||
|- | |- | ||
- | |align="center"| | + | |align="center"|L122 |
- | |align="center"| | + | |align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI) |
- | + | |align="center"|Amp | |
- | <br> | + | |align="center"|4 |
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |
- | |align="center"| | + | |
|align="center"|OK | |align="center"|OK | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
|- | |- | ||
|align="center"|L123 | |align="center"|L123 | ||
- | |align="center"| | + | |align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI) |
- | RBS lasI - | + | |align="center"|Amp |
- | <br>D107 (BV) - D131 (BI) | + | |align="center"|> 4000 |
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |align="center"|OK |
- | |align="center"| | + | |
- | |align="center"| | + | |
|- | |- | ||
- | |align="center"| | + | |align="center"|L126 |
- | |align="center"| | + | |align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI) |
- | Strongest RBS | + | |align="center"|Amp |
- | + | |align="center"|944 | |
- | + | |align="center"|No | |
- | + | |align="center"|OK | |
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | + | ||
- | <br>D102 (BV) - | + | |
- | |align="center"| | + | |
- | |align="center"| | + | |
- | |align="center"| | + | |
- | |align="center"| | + | |
|} | |} | ||
+ | |||
+ | [[Image:transfo.jpg|frame|center|L101]] | ||
+ | ==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |
Latest revision as of 17:09, 13 August 2008
Transformation of 1st AugustResults of the transformation
==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |