Team:Paris/August 2

From 2008.igem.org

(Difference between revisions)

Latest revision as of 17:09, 13 August 2008

Transformation of 1st August

Results of the transformation

Ligation name	Description	Antibio	Nb colonies observed	Fluorescence	Comments
puc19	positive control	Amp		-	OK
L100	rbs TetR - ECFP D110 (BV) - D130 (BI)	Amp	608	No	OK
L101	rbs TetR - GFP tripart D110 (BV) - D131 (BI)	Amp	> 4000	GFP positive	OK
L113	AracpBAD - ecfp D126 (BV) - D130 (BI)	Kana	9
L114	AracpBAD - gfp tripart D126 (BV) - D131 (BI)	Kana	9
L120	tetR repressible promoter - ECFP D106 (BV) - D130 (BI)	Amp	74
L122	RBS-lasI - ecfp D107 (BV) - D130 (BI)	Amp	4	No	OK
L123	RBS lasI - GFP tripart D107 (BV) - D131 (BI)	Amp	> 4000	No	OK
L126	Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI)	Amp	944	No	OK

L101

==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed.

@@ Line 1: / Line 1: @@
-{{Paris/Calendar_Links|August 1 |August 03}}
+{{Paris/Calendar_Links|August 1|August 3}}
+== Transformation of 1st August ==
 === Results of the transformation ===
@@ Line 12: / Line 16: @@
 |align="center"|'''Comments'''
 |-
-|align="center"|L100
+|align="center"|puc19
-|align="center"|rbs TetR - ECFP
+|align="center"|positive control
-<br>D110 (BV) -  D130 (BI)
+|align="center"|Amp
-|align="center"|
-|align="center"|0
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L101
-|align="center"|rbs TetR - GFP tripart
-<br>D110 (BV) -  D131 (BI)
-|align="center"|
-|align="center"|(1)
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L102
-|align="center"|Strong rbs - YFP
-<br>D129 (BV) -  D118 (BI)
-|align="center"|
-|align="center"|+/- 100
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L103
-|align="center"|Strong rbs - GFP
-<br>D129 (BV) -  D122 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L104
-|align="center"|Strong rbs - lasR activator
-<br>D129 (BV) -  D114 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L105
-|align="center"|
-Strong promoter - ECFP
-<br>D123 (BV) - D130 (BI)
-|align="center"|
-|align="center"|a lot ++
 |align="center"|
+|align="center"|-
 |align="center"|OK
-|-
-|align="center"|L106
-|align="center"|
-Strong promoter - gfp Tripart
-<br>D123 (BV) - D131 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|OK
-|-
-|align="center"|L107
-|align="center"|
-Strongest promoter - ECFP
-<br>D103 (BV) - D131 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L108 n°2 (the right one)
-|align="center"|
-Strong promoter -  gfp Tripart
-<br>D103 (BV) - D130 (BI)
-|align="center"|
-|align="center"|+/- 100
-|align="center"|
-|align="center"|OK
-|-
-|align="center"|L109 to complete
-|align="center"|
-Strong promoter -  ecfp
-<br>D124 (BV) - D130 (BI)
-|align="center"|
-|align="center"|+/- 100
-|align="center"|
-|align="center"|OK
-|-
-|align="center"|L110
-|align="center"|
-Medium promoter - gfp Tripart
-<br>D124 (BV) - D131 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|OK
-|-
-|align="center"|L111
-|align="center"|
-Weak promoter - ECFP
-<br>D104 (BV) - D130 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L112
-|align="center"|
-Weak promoter - gfp
-<br>D104 (BV) - D131 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L113
-|align="center"|
-AracpBAD - ecfp
-<br>D126 (BV) - D130 (BI)
-|align="center"|
-|align="center"|1
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L114
-|align="center"|
-AracpBAD - gfp tripart
-<br>D126 (BV) - D131 (BI)
-|align="center"|
-|align="center"|2
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L115
-|align="center"|
-pLas - ECFP
-<br>D105 (BV) - D130 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L116
-|align="center"|
-pLas - gfp Tripart
-<br>D105 (BV) - D131 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L117
-|align="center"|
-yfp - Double Terminator
-<br>D125 (FV) - D117 (FI)
-|align="center"|
-|align="center"|+/- 30
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L118
-|align="center"|
-rfp - Double Terminator
-<br>D125 (FV) - D121 (FI)
-|align="center"|
-|align="center"|+/- 30
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L119
-|align="center"|
-lasR activator + LVA - Double Terminator
-<br>D125 (FV) - D113 (FI)
-|align="center"|
-|align="center"|+/- 30
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L120
-|align="center"|
-tetR repressible - ECFP
-<br>D106 (BV) - D130 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L121
-|align="center"|
-Strong promoter - gfp tripart
-<br>D106 (BV) - D130 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|OK
-|-
-|align="center"|L122
-|align="center"|
-RBS-lasI - ecfp
-<br>D107 (BV) - D130 (BI)
-|align="center"|
-|align="center"|0
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L123
-|align="center"|
-RBS lasI - ecfp
-<br>D107 (BV) - D131 (BI)
-|align="center"|
-|align="center"|1
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L124
-|align="center"|
-Strongest RBS - rfp
-<br>D102 (BV) - D122 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L125
-|align="center"|
-Strongest RBS - yfp
-<br>D102 (BV) - D118 (BI)
-|align="center"|
-|align="center"|a lot ++
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L126
-|align="center"|
-Strongest RBS (1)- LacR activator (+LVA)
-<br>D102 (BV) - D118 (BI)
-|align="center"|
-|align="center"|no dish found!!!
-|align="center"|
-|align="center"|To do again
-|-
-|align="center"|L127
-|align="center"|
-gfp (1)- Double terminator
-<br>D119 (BV) - D125 (BI)
-|align="center"|
-|align="center"|no dish found!!!
-|align="center"|
-|align="center"|To do again
-|}
-=== Results of the transformation (bis-the good one to leave in august 2)===
-{| border="1"
-|align="center"|'''Ligation name'''
-|align="center"|'''Description'''
-|align="center"|'''Antibio'''
-|align="center"|'''Nb colonies observed'''
-|align="center"|'''Fluorescence'''
-|align="center"|'''Comments'''
 |-
 |align="center"|L100
-|align="center"|
+|align="center"|rbs TetR - ECFP<br>D110 (BV) -  D130 (BI)
-rbs TetR - ECFP
-<br>D110 (BV) -  D130 (BI)
 |align="center"|Amp
 |align="center"|608
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |-
 |align="center"|L101
-|align="center"|
+|align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI)
-rbs TetR - GFP tripart
-<br>D110 (BV) -  D131 (BI)
 |align="center"|Amp
 |align="center"|> 4000
@@ Line 290: / Line 38: @@
 |-
 |align="center"|L113
-|align="center"|
+|align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI)
-AracpBAD - ecfp
-<br>D126 (BV) - D130 (BI)
 |align="center"|Kana
 |align="center"|9
 |align="center"|
-|align="center"|To do again
+|align="center"|
 |-
 |align="center"|L114
-|align="center"|
+|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
-AracpBAD - gfp tripart
-<br>D126 (BV) - D131 (BI)
 |align="center"|Kana
 |align="center"|9
 |align="center"|
-|align="center"|To do again
+|align="center"|
 |-
 |align="center"|L120
-|align="center"|
+|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
-tetR repressible promoter - ECFP
-<br>D106 (BV) - D130 (BI)
 |align="center"|Amp
 |align="center"|74
 |align="center"|
-|align="center"|To do again
+|align="center"|
 |-
 |align="center"|L122
-|align="center"|
+|align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI)
-RBS-lasI - ecfp
-<br>D107 (BV) - D130 (BI)
 |align="center"|Amp
 |align="center"|4
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |-
 |align="center"|L123
-|align="center"|
+|align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI)
-RBS lasI - ecfp
-<br>D107 (BV) - D131 (BI)
 |align="center"|Amp
 |align="center"|> 4000
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |-
 |align="center"|L126
-|align="center"|
+|align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
-Strongest RBS (1)- LasR activator (+LVA)
-<br>D102 (BV) - D114 (BI)
 |align="center"|Amp
 |align="center"|944
-|align="center"|
+|align="center"|No
-|align="center"|To do again
+|align="center"|OK
 |}
+[[Image:transfo.jpg|frame|center|L101]]
+==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed.