Team:Paris/August 2
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- | {{Paris/Calendar_Links|August 1 |August | + | {{Paris/Calendar_Links|August 1|August 3}} |
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+ | == Transformation of 1st August == | ||
=== Results of the transformation === | === Results of the transformation === | ||
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|align="center"|'''Comments''' | |align="center"|'''Comments''' | ||
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- | |align="center"| | + | |align="center"|puc19 |
- | |align="center"| | + | |align="center"|positive control |
- | + | |align="center"|Amp | |
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|align="center"|OK | |align="center"|OK | ||
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|- | |- | ||
|align="center"|L100 | |align="center"|L100 | ||
- | |align="center"| | + | |align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI) |
- | rbs TetR - ECFP | + | |
- | <br>D110 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|608 | |align="center"|608 | ||
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |align="center"|OK |
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|align="center"|L101 | |align="center"|L101 | ||
- | |align="center"| | + | |align="center"|rbs TetR - GFP tripart<br>D110 (BV) - D131 (BI) |
- | rbs TetR - GFP tripart | + | |
- | <br>D110 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|> 4000 | |align="center"|> 4000 | ||
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|align="center"|L113 | |align="center"|L113 | ||
- | |align="center"| | + | |align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI) |
- | AracpBAD - ecfp | + | |
- | <br>D126 (BV) - D130 (BI) | + | |
|align="center"|Kana | |align="center"|Kana | ||
|align="center"|9 | |align="center"|9 | ||
|align="center"| | |align="center"| | ||
- | |align="center"| | + | |align="center"| |
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|align="center"|L114 | |align="center"|L114 | ||
- | |align="center"| | + | |align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI) |
- | AracpBAD - gfp tripart | + | |
- | <br>D126 (BV) - D131 (BI) | + | |
|align="center"|Kana | |align="center"|Kana | ||
|align="center"|9 | |align="center"|9 | ||
|align="center"| | |align="center"| | ||
- | |align="center"| | + | |align="center"| |
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|align="center"|L120 | |align="center"|L120 | ||
- | |align="center"| | + | |align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI) |
- | tetR repressible promoter - ECFP | + | |
- | <br>D106 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|74 | |align="center"|74 | ||
|align="center"| | |align="center"| | ||
- | |align="center"| | + | |align="center"| |
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|align="center"|L122 | |align="center"|L122 | ||
- | |align="center"| | + | |align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI) |
- | RBS-lasI - ecfp | + | |
- | <br>D107 (BV) - D130 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|4 | |align="center"|4 | ||
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |align="center"|OK |
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|align="center"|L123 | |align="center"|L123 | ||
- | |align="center"| | + | |align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI) |
- | RBS lasI - | + | |
- | <br>D107 (BV) - D131 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|> 4000 | |align="center"|> 4000 | ||
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |align="center"|OK |
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|align="center"|L126 | |align="center"|L126 | ||
- | |align="center"| | + | |align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI) |
- | Strongest RBS (1)- LasR activator (+LVA) | + | |
- | <br>D102 (BV) - D114 (BI) | + | |
|align="center"|Amp | |align="center"|Amp | ||
|align="center"|944 | |align="center"|944 | ||
- | |align="center"| | + | |align="center"|No |
- | |align="center"| | + | |align="center"|OK |
|} | |} | ||
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+ | [[Image:transfo.jpg|frame|center|L101]] | ||
+ | ==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |
Latest revision as of 17:09, 13 August 2008
Transformation of 1st AugustResults of the transformation
==> Conclusion:Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |