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| - | {{Paris/Calendar_Links|August 1 |August 3}} | + | {{Paris/Calendar_Links|August 1|August 3}} |
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| | + | == Transformation of 1st August == |
| | | | |
| | === Results of the transformation === | | === Results of the transformation === |
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| | |align="center"|'''Comments''' | | |align="center"|'''Comments''' |
| | |- | | |- |
| - | |align="center"|L100 | + | |align="center"|puc19 |
| - | |align="center"| | + | |align="center"|positive control |
| - | rbs TetR - ECFP
| + | |align="center"|Amp |
| - | <br>D110 (BV) - D130 (BI)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|0
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|To do again
| + | |
| - | |-
| + | |
| - | |align="center"|L101
| + | |
| - | |align="center"|
| + | |
| - | rbs TetR - GFP tripart
| + | |
| - | <br>D110 (BV) - D131 (BI)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|(1)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|To do again
| + | |
| - | |-
| + | |
| - | |align="center"|L102
| + | |
| - | |align="center"|
| + | |
| - | Strong rbs - YFP
| + | |
| - | <br>D129 (BV) - D118 (BI)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|+/- 100
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|To do again
| + | |
| - | |-
| + | |
| - | |align="center"|L103
| + | |
| - | |align="center"|
| + | |
| - | Strong rbs - GFP
| + | |
| - | <br>D129 (BV) - D122 (BI)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|a lot ++
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|To do again
| + | |
| - | |-
| + | |
| - | |align="center"|L104
| + | |
| - | |align="center"|
| + | |
| - | Strong rbs - lasR activator
| + | |
| - | <br>D129 (BV) - D114 (BI)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|a lot ++
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|To do again
| + | |
| - | |-
| + | |
| - | |align="center"|L105
| + | |
| - | |align="center"|
| + | |
| - | Strong promoter - ECFP
| + | |
| - | <br>D123 (BV) - D130 (BI)
| + | |
| - | |align="center"|
| + | |
| - | |align="center"|a lot ++ | + | |
| | |align="center"| | | |align="center"| |
| | + | |align="center"|- |
| | |align="center"|OK | | |align="center"|OK |
| - | |-
| |
| - | |align="center"|L106
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| - | |align="center"|
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| - | Strong promoter - gfp Tripart
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| - | <br>D123 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|OK
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| - | |-
| |
| - | |align="center"|L107
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| - | |align="center"|
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| - | Strongest promoter - ECFP
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| - | <br>D103 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L108 n°2 (the right one)
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| - | |align="center"|
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| - | Strong promoter - gfp Tripart
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| - | <br>D103 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|+/- 100
| |
| - | |align="center"|
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| - | |align="center"|OK
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| - | |-
| |
| - | |align="center"|L109
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| - | |align="center"|
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| - | Strong promoter - ecfp
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| - | <br>D124 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|+/- 100
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| - | |align="center"|
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| - | |align="center"|OK
| |
| - | |-
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| - | |align="center"|L110
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| - | |align="center"|
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| - | Medium promoter - gfp Tripart
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| - | <br>D124 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|OK
| |
| - | |-
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| - | |align="center"|L111
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| - | |align="center"|
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| - | Weak promoter - ECFP
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| - | <br>D104 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L112
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| - | |align="center"|
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| - | Weak promoter - gfp
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| - | <br>D104 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L113
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| - | |align="center"|
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| - | AracpBAD - ecfp
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| - | <br>D126 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|1
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L114
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| - | |align="center"|
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| - | AracpBAD - gfp tripart
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| - | <br>D126 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|2
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L115
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| - | |align="center"|
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| - | pLas - ECFP
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| - | <br>D105 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L116
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| - | |align="center"|
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| - | pLas - gfp Tripart
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| - | <br>D105 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L117
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| - | |align="center"|
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| - | yfp - Double Terminator
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| - | <br>D117 (FI) - D125 (FV)
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| - | |align="center"|
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| - | |align="center"|+/- 30
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L118
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| - | |align="center"|
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| - | rfp - Double Terminator
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| - | <br>D121 (FI) - D125 (FV)
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| - | |align="center"|
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| - | |align="center"|+/- 30
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L119
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| - | |align="center"|
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| - | lasR activator + LVA - Double Terminator
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| - | <br>D113 (FI) - D125 (FV)
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| - | |align="center"|
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| - | |align="center"|+/- 30
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L120
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| - | |align="center"|
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| - | tetR repressible - ECFP
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| - | <br>D106 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L121
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| - | |align="center"|
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| - | Strong promoter - gfp tripart
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| - | <br>D106 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|OK
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| - | |-
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| - | |align="center"|L122
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| - | |align="center"|
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| - | RBS-lasI - ecfp
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| - | <br>D107 (BV) - D130 (BI)
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| - | |align="center"|
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| - | |align="center"|0
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L123
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| - | |align="center"|
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| - | RBS lasI - ecfp
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| - | <br>D107 (BV) - D131 (BI)
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| - | |align="center"|
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| - | |align="center"|1
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L124
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| - | |align="center"|
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| - | Strongest RBS - rfp
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| - | <br>D102 (BV) - D122 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L125
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| - | |align="center"|
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| - | Strongest RBS - yfp
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| - | <br>D102 (BV) - D118 (BI)
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| - | |align="center"|
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| - | |align="center"|a lot ++
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L126
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| - | |align="center"|
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| - | Strongest RBS (1)- LacR activator (+LVA)
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| - | <br>D102 (BV) - D118 (BI)
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| - | |align="center"|
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| - | |align="center"|no dish found!!!
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |-
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| - | |align="center"|L127
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| - | |align="center"|
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| - | gfp (1)- Double terminator
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| - | <br>D119 (FI) - D125 (FV)
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| - | |align="center"|
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| - | |align="center"|no dish found!!!
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| - | |align="center"|
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| - | |align="center"|To do again
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| - | |}
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| - |
| |
| - | === Results of the transformation (bis-the good one to leave in august 2)===
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| - |
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| - |
| |
| - | {| border="1"
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| - | |align="center"|'''Ligation name'''
| |
| - | |align="center"|'''Description'''
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| - | |align="center"|'''Antibio'''
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| - | |align="center"|'''Nb colonies observed'''
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| - | |align="center"|'''Fluorescence'''
| |
| - | |align="center"|'''Comments'''
| |
| | |- | | |- |
| | |align="center"|L100 | | |align="center"|L100 |
| - | |align="center"| | + | |align="center"|rbs TetR - ECFP<br>D110 (BV) - D130 (BI) |
| - | rbs TetR - ECFP | + | |
| - | <br>D110 (BV) - D130 (BI) | + | |
| | |align="center"|Amp | | |align="center"|Amp |
| | |align="center"|608 | | |align="center"|608 |
| - | |align="center"| | + | |align="center"|No |
| - | |align="center"|To do again | + | |align="center"|OK |
| | |- | | |- |
| | |align="center"|L101 | | |align="center"|L101 |
| - | |align="center"| | + | |align="center"|rbs TetR - GFP tripart<br>D110 (BV) - D131 (BI) |
| - | rbs TetR - GFP tripart | + | |
| - | <br>D110 (BV) - D131 (BI) | + | |
| | |align="center"|Amp | | |align="center"|Amp |
| | |align="center"|> 4000 | | |align="center"|> 4000 |
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| | |- | | |- |
| | |align="center"|L113 | | |align="center"|L113 |
| - | |align="center"| | + | |align="center"|AracpBAD - ecfp<br>D126 (BV) - D130 (BI) |
| - | AracpBAD - ecfp | + | |
| - | <br>D126 (BV) - D130 (BI) | + | |
| | |align="center"|Kana | | |align="center"|Kana |
| | |align="center"|9 | | |align="center"|9 |
| | |align="center"| | | |align="center"| |
| - | |align="center"|To do again | + | |align="center"| |
| | |- | | |- |
| | |align="center"|L114 | | |align="center"|L114 |
| - | |align="center"| | + | |align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI) |
| - | AracpBAD - gfp tripart | + | |
| - | <br>D126 (BV) - D131 (BI) | + | |
| | |align="center"|Kana | | |align="center"|Kana |
| | |align="center"|9 | | |align="center"|9 |
| | |align="center"| | | |align="center"| |
| - | |align="center"|To do again | + | |align="center"| |
| | |- | | |- |
| | |align="center"|L120 | | |align="center"|L120 |
| - | |align="center"| | + | |align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI) |
| - | tetR repressible promoter - ECFP | + | |
| - | <br>D106 (BV) - D130 (BI) | + | |
| | |align="center"|Amp | | |align="center"|Amp |
| | |align="center"|74 | | |align="center"|74 |
| | |align="center"| | | |align="center"| |
| - | |align="center"|To do again | + | |align="center"| |
| | |- | | |- |
| | |align="center"|L122 | | |align="center"|L122 |
| - | |align="center"| | + | |align="center"|RBS-lasI - ecfp<br>D107 (BV) - D130 (BI) |
| - | RBS-lasI - ecfp | + | |
| - | <br>D107 (BV) - D130 (BI) | + | |
| | |align="center"|Amp | | |align="center"|Amp |
| | |align="center"|4 | | |align="center"|4 |
| - | |align="center"| | + | |align="center"|No |
| - | |align="center"|To do again | + | |align="center"|OK |
| | |- | | |- |
| | |align="center"|L123 | | |align="center"|L123 |
| - | |align="center"| | + | |align="center"|RBS lasI - GFP tripart<br>D107 (BV) - D131 (BI) |
| - | RBS lasI - ecfp | + | |
| - | <br>D107 (BV) - D131 (BI) | + | |
| | |align="center"|Amp | | |align="center"|Amp |
| | |align="center"|> 4000 | | |align="center"|> 4000 |
| - | |align="center"| | + | |align="center"|No |
| - | |align="center"|To do again | + | |align="center"|OK |
| | |- | | |- |
| | |align="center"|L126 | | |align="center"|L126 |
| - | |align="center"| | + | |align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI) |
| - | Strongest RBS (1)- LasR activator (+LVA) | + | |
| - | <br>D102 (BV) - D114 (BI) | + | |
| | |align="center"|Amp | | |align="center"|Amp |
| | |align="center"|944 | | |align="center"|944 |
| - | |align="center"| | + | |align="center"|No |
| - | |align="center"|To do again | + | |align="center"|OK |
| | |} | | |} |
| | + | |
| | + | [[Image:transfo.jpg|frame|center|L101]] |
| | + | ==> '''Conclusion:'''Tomorrow we will do a screening PCR on the only Ligation transformant succeed. |
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