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Team:Paris/August 14
From 2008.igem.org
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=='''Results of the PCR of the wonderful promoter of ''fliL'' '''== | =='''Results of the PCR of the wonderful promoter of ''fliL'' '''== | ||
[[Image:KR000147.jpg|thumb|Amplification of pfliL]] | [[Image:KR000147.jpg|thumb|Amplification of pfliL]] | ||
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Electrophoresis settings : | Electrophoresis settings : | ||
*Gel 1.5% agar | *Gel 1.5% agar | ||
*Ladder 100 bp | *Ladder 100 bp | ||
*Volume of template : 3 µL | *Volume of template : 3 µL | ||
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Revision as of 11:18, 15 August 2008
Results of the PCR of the wonderful promoter of fliL
Electrophoresis settings :
- Gel 1.5% agar
- Ladder 100 bp
- Volume of template : 3 µL
| Name | Promotor | Band | Expected size | Measured size |
| PCR_135 | pfliL | 2 | 197 bp | |
| PCR_135' | Negative Control | 3 | 0 bp |
Digestion and Ligation of the wonderful promoter of fliL
As the primer used to amplify the promoter of fliL had only two nucleotides after the
