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Team:Paris/August 14
From 2008.igem.org
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(→Results of the PCR of the wonderful promoter of fliL) |
(→Results of the PCR of the wonderful promoter of fliL) |
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|'''Name''' | |'''Name''' | ||
|'''Promotor''' | |'''Promotor''' | ||
| - | |align="center"|''' | + | |align="center"|'''Well''' |
|align="center"|'''Expected size''' | |align="center"|'''Expected size''' | ||
|align="center"|'''Measured size''' | |align="center"|'''Measured size''' | ||
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|style="background: #cbff7B"|<center> 0 bp </center> | |style="background: #cbff7B"|<center> 0 bp </center> | ||
|} | |} | ||
| + | |||
| + | Remarks: | ||
| + | We observe that the bands are curved, we suppose that the wells were not very clean. | ||
| + | The size of fliL is good, we will digest it and ligate it today. | ||
=='''Digestion and Ligation of the wonderful promoter of ''fliL'' '''== | =='''Digestion and Ligation of the wonderful promoter of ''fliL'' '''== | ||
As the primer used to amplify the promoter of ''fliL'' had only two nucleotides after the | As the primer used to amplify the promoter of ''fliL'' had only two nucleotides after the | ||
Revision as of 11:21, 15 August 2008
Results of the PCR of the wonderful promoter of fliL
Electrophoresis settings :
- Gel 1.5% agar
- Ladder 100 bp
- Volume of template : 3 µL
| Name | Promotor | Well | Expected size | Measured size |
| PCR_135 | pfliL | 2 | 197 bp | |
| PCR_135' | Negative Control | 3 | 0 bp |
Remarks: We observe that the bands are curved, we suppose that the wells were not very clean. The size of fliL is good, we will digest it and ligate it today.
Digestion and Ligation of the wonderful promoter of fliL
As the primer used to amplify the promoter of fliL had only two nucleotides after the
