Team:Hawaii/Milestone

From 2008.igem.org

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{{Team:Hawaii/Header}}[[Image:20080612-work breakdown structure.jpg|thumb|300px|right]]
== Immediate ==
== Immediate ==
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* [[Team:Hawaii/Meeting/2008-05-29|2008.05.29]] Presenation. Grace: Other parts.
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<onlyinclude>
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* Present proposal for part B: the idea you are championing
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* Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
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*: Thu. May 29th (?/?)
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* Express GFP constructs in ''Synechocystis''
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*: Thu. Jun 5th (?/?)
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</onlyinclude>
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* Culturing Protocol Optimization (KS)
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== Near Term ==
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* Create competent DH5a E. coli stocks (?)
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* Plasmid Transformation Plan/Protocol (GK/MR)
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* Plasmid Transformation (GK/MR)
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== Long Term ==
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* [[Team:Hawaii/Meeting/2008-06-27|2008.06.27]] Make final decision on Part B of our iGEM project
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== Completed Milestones ==
== Completed Milestones ==
* 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
* 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
* [[Team:Hawaii/Notebook/2008-05-22|2008.05.24]] Create seed stocks for test transformation (NW/MR)
* [[Team:Hawaii/Notebook/2008-05-22|2008.05.24]] Create seed stocks for test transformation (NW/MR)
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* [[Team:Hawaii/Meeting/2008-05-22|2008.05.22]] Describe, present [http://partsregistry.org/Part_Types part types]. 1.  Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids
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* [[Team:Hawaii/Meeting/2008-05-22|2008.05.22]] Describe, present [http://partsregistry.org/Part_Types part types]. 1.  Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
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* 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
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<!-- Team Hawaii Tracker -->
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* 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
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<html>
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* 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
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<script type="text/javascript">
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* 2008.06.04 GK and KS are now autoclave trained
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var gaJsHost = (("https:" == document.location.protocol) ? "https://ssl." : "http://www.");
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* 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table.
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document.write(unescape("%3Cscript src='" + gaJsHost + "google-analytics.com/ga.js' type='text/javascript'%3E%3C/script%3E"));
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* 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated)
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</script>
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* 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle.  linear DNA need recombination.  Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR)
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<script type="text/javascript">
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* 2008.06.12 Final draft of proposal due
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var pageTracker = _gat._getTracker("UA-308155-6");
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* 2008.06.23 Transform PCC6803 w/ pRL1383a
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pageTracker._initData();
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* 2008.06.24 First batch of primers ordered
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pageTracker._trackPageview();
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* 2008.06.24 Biobrick parts list completed
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</script>
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* 2008.06.24 RP1 and RP4 oriT are the same
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</html>
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* 2008.08.13 Replaced pRL1383a MCS with BioBrick MCS (GK)
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<!-- Team Hawaii Tracker -->
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{{Team:Hawaii/Footer}}

Latest revision as of 17:35, 15 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)
20080612-work breakdown structure.jpg

Immediate

  • Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
  • Express GFP constructs in Synechocystis

Near Term

Completed Milestones

  • 2008.05.xx figure out OD Reading operation: Beckman 640U spectrophotometer (AB/NW)
  • 2008.05.24 Create seed stocks for test transformation (NW/MR)
  • 2008.05.22 Describe, present [http://partsregistry.org/Part_Types part types]. 1. Norman: biobrick assembly, parts, 2. Margaret: devices, 3. Krystle: chassis & plasmids, 4. Grace: other
  • 2008.06.01 Plasmid Transformation Plan/Protocol (GK/MR)
  • 2008.06.02 Create competent DH5a E. coli stocks (GK/MR/NW)
  • 2008.06.03-2008.06.04 Plasmid Transformation, document results (GK/NW)
  • 2008.06.04 GK and KS are now autoclave trained
  • 2008.06.10 Fluorometer found (in Dr. Su's lab), 470nm blue LED lights purchased for constructing our own light table.
  • 2008.06.11 First draft Work Breakdown Structure diagram drawn on whiteboard, detailing all deliverables. (please keep it updated)
  • 2008.06.11 Does RSF1010 replicate autonomously in PCC6803? YES, only when it goes in cell as circle. linear DNA need recombination. Determined we need to test all three methods to find one that works: 1. Chemical 2. Electroporation 3. Conjugation (MR)
  • 2008.06.12 Final draft of proposal due
  • 2008.06.23 Transform PCC6803 w/ pRL1383a
  • 2008.06.24 First batch of primers ordered
  • 2008.06.24 Biobrick parts list completed
  • 2008.06.24 RP1 and RP4 oriT are the same
  • 2008.08.13 Replaced pRL1383a MCS with BioBrick MCS (GK)

[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]

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