Team:Paris/August 19
From 2008.igem.org
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AnaJimenez (Talk | contribs) (→Ligation) |
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|'''Insert volume''' | |'''Insert volume''' | ||
|'''Product description''' | |'''Product description''' | ||
+ | |'''Antibiotic''' | ||
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|L155 | |L155 | ||
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|J23101 promoter-gfp generator | |J23101 promoter-gfp generator | ||
+ | |Amp | ||
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|pTet promoter-gfp generator | |pTet promoter-gfp generator | ||
+ | |Kana | ||
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|Vector autoligation control | |Vector autoligation control | ||
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|L157 | |L157 | ||
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|tetR | |tetR | ||
|tetR-B0015 | |tetR-B0015 | ||
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Revision as of 14:44, 21 August 2008
Screening of the cloning of OmpR*, EnvZ* and FlhDC+promotorElectrophoresis
Minipreps and glycerol stock
Screening of the cloning of E0240 and FlhDC+promotorSpreading the clones in order to obtain single colonies
The PCR screening of the transformants L139 and L142 of august 15th revealed several bands for a given clone including one band appearing at the right size.
In order to check these 2 hypothesis and to isolate (if it is possible) the right clone (containing the plasmid with the insert). We decided to spread the "clone" in question in a LB plate in order to carry out a PCR screening on single colonies.
Promoter characterization plasmidsLigation
TransformationDigestionMeasurement of concentration of miniprepsto be modified standard protocol
Digestionto be modified
We had a problem with a gel and we lost these digestions. |