Team:KULeuven/Freezer

From 2008.igem.org

(Difference between revisions)
(Parts stored in the fridge in the lab (4°C))
(Parts stored in the fridge in the lab (4°C))
Line 209: Line 209:
|[http://partsregistry.org/Part:BBa_F1610 F1610]|| ''Eco''R I||''Xba'' I||Vector||3956||26||4.5||35||August 19
|[http://partsregistry.org/Part:BBa_F1610 F1610]|| ''Eco''R I||''Xba'' I||Vector||3956||26||4.5||35||August 19
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
-
|[http://partsregistry.org/Part:BBa_K1450151 K145151]|| ''Eco''R I||''Spe'' I||Insert||306||25||25.2||36||August 19
+
|[http://partsregistry.org/Part:BBa_K1450151 K145151]|| ''Eco''R I||''Spe'' I||Insert||306||19||25.2||36||August 19
|- style="background: #E0FFFF; text-align: center;"
|- style="background: #E0FFFF; text-align: center;"
|[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Xba'' I||Vector||13||2092||17.6||25||August 19  
|[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Xba'' I||Vector||13||2092||17.6||25||August 19  
Line 243: Line 243:
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||144||+/-28||2.4||42||August 25
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032]|| ''Eco''R I||''Spe'' I||Insert||144||+/-28||2.4||42||August 25
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||774||24||27.7||43||August 25
+
|[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2]|| ''Eco''R I||''Spe'' I||Plasmid||2056||24||27.7||43||August 25
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
-
|[http://partsregistry.org/Part:BBa_K145001 K145001]|| ''Eco''R I||''Spe'' I||Insert||2730||+/-30||24.3||24||August 22
+
|[http://partsregistry.org/Part:BBa_K145001 K145001]|| ''Eco''R I||''Spe'' I||Insert||2730||+/-21||24.3||24||August 22
|- style="background: #E0FFFF; text-align: center;"   
|- style="background: #E0FFFF; text-align: center;"   
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||432||+/-30||4.6||34||August 22
|[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]|| ''Eco''R I||''Spe'' I||Insert||432||+/-30||4.6||34||August 22

Revision as of 08:33, 26 August 2008

  dock/undock dropdown  


Parts stored in the fridge in the lab (4°C)

Plasmids: MiniPrep Products
Part Plasmid Description µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 4050.41July 23
[http://partsregistry.org/Part:BBa_M30109 M30109]pSB1AC3Light-sensor+/- 3054.32July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 60345.03 July 24
[http://partsregistry.org/Part:BBa_J23100 J23100]BBa_J61002 (Ap)Constitutive promoter+/- 30176.14 July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 2073.85July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 3095.16July 24
[http://partsregistry.org/Part:BBa_B0034 B0034]pSB1A2RBS (efficiency 1)+/- 40104.07July 29
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 25177.18July 24
[http://partsregistry.org/Part:BBa_C0040 C0040]pSB1A2TetR repressor+/- 30161.79July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 4063.010July 23
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 10112.311July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 20130.312July 24
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 43303.113July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 27.5213.614July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 48311.031July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 30214.047July 29
[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Terminator+/- 40209.548July 29
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 4032.015July 23
[http://partsregistry.org/Part:BBa_R0084 R0084]pSB1A2OmpF promoter+/- 3060.916July 24
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4084.219July 23
[http://partsregistry.org/Part:BBa_E0022 E0022]pSB1A2eCFP+/- 4086.420July 24
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI+/- 30133.121July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40146.449July 29
[http://partsregistry.org/Part:BBa_C0012 C0012]pSB1A2LacI repressor+/- 40203.150July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 35117.423July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 39.5144.824July 29
[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)3d lock+/- 48129.128July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 40160.725July 29
[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)3d key+ terminator+/- 46308.329July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 46375.126July 29
[http://partsregistry.org/Part:BBa_J23109 J23109]BBa_J61002 (Ap)Constitutive Promoter+/- 4840430July 29
[http://partsregistry.org/Part:BBa_I712074 I712074]BBa_J52017 (Ap,Km)T7 Promotor+/- 42.5208.227July 29
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3RBS + LuxI + Terminator+/- 30104.022July 29
[http://partsregistry.org/Part:BBa_I712022 I712022]BBa_J52017 (Km, Ap)T7 polymerase+/- 40394.135July 31
[http://partsregistry.org/Part:BBa_R0062 R0062]pSB1A2LuxR regulated promoter +/- 40104.137July 31
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter+/- 4564.043July 29
[http://partsregistry.org/Part:BBa_R0011 R0011]pSB1A2LacI regulated promoter +/- 4069.844July 29
[http://partsregistry.org/Part:BBa_I12006 I12006]pSB2K3434 cI repressible promoter +/- 4825.851August 08
[http://partsregistry.org/Part:BBa_I12006 I12006]pSB2K3434 cI repressible promoter +/- 4852.752August 08
[http://partsregistry.org/Part:BBa_J23116 J23116]BBa_J61002 (Ap)Constitutive promoter +/- 48328.853August 08
[http://partsregistry.org/Part:BBa_C0060 C0060]pSB1A2lactonase production+/- 4884.260August 11
[http://partsregistry.org/Part:BBa_C0062 C0062]pSB1A2LuxR repressor+/- 4876.261August 11
[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2RBS(medium)+/- 551.062August 11
[http://partsregistry.org/Part:BBa_R0040 R0040]pSB1A2tetR repressible promoter+/- 4837.963August 11
[http://partsregistry.org/Part:BBa_P1010 P1010]-CcdB gene+/- 5051.564August 12
[http://partsregistry.org/Part:BBa_P1010 P1010]-CcdB gene+/- 5062.865August 12
[http://partsregistry.org/Part:BBa_P1010 P1010]-CcdB gene+/- 50297.233July 31
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3HSL Sender Device+/- 5057.666August 12
[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3HSL Sender Device+/- 4053.167August 12
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 46124.854August 08
[http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:pSB1A2 pSB1A2]pSB1A2GFP with LVA tag+/- 46152.755August 08
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 4689.957August 08
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 4694.458August 08
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Output+/- 46151.859August 08
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 20150.239August 20
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]BBa_J23006 (Ap)Ligation for Filter+/- 2058.340August 20
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)Ligation for Filter+/- 1076.141August 20
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022]BBa_J23006 (Ap)Ligation for Filter+/- 2061.142August 20
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 508.717August 21
[http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015]pSB1AK3Ligation for Cell Death+/- 5021.045August 21
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Input+/- 40102.518August 25
[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_F1610 F1610]pSB1AK3Ligation for Inverter+/- 1358.828August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for output+/- 3064.435August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Output+/- 3593.163August 25
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032]pSB1A2Ligation for Input+/- 3596.256August 25


Restriction Digests
Part Enzyme 1 Enzyme 2 vector/insert length (bp) µl left ng/µl Box Date
[http://partsregistry.org/Part:BBa_J23100 J23100]EcoR ISpe IInsert5238.12.61July 24
[http://partsregistry.org/Part:BBa_C0040 C0040] EcoR ISpe IInsert677187.12July 24
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert12533.43.83July 24
[http://partsregistry.org/Part:BBa_E0022 E0022] EcoR ISpe IInsert779229.54July 24
[http://partsregistry.org/Part:BBa_J23109 J23109] EcoR ISpe IInsert52214.25July 29
[http://partsregistry.org/Part:BBa_R0084 R0084] EcoR ISpe IInsert125256.16July 29
[http://partsregistry.org/Part:BBa_I712074 I712074] EcoR ISpe IInsert62233.47July 29
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090212.98July 30
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3090451.39July 31
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3308255.122August 07
[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector33031111.711July 31
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert803285.420August 12
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72208.512July 30
[http://partsregistry.org/Part:BBa_R0011 R0011] EcoR ISpe IInsert72207.513July 30
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert7741824.714August 01
[http://partsregistry.org/Part:BBa_C0060 C0060] EcoR ISpe IInsert80698.215August 01
[http://partsregistry.org/wiki/index.php?title=Part:pSB1A2 pSB1A2] EcoR ISpe IPlasmid2079918.216August 01
[http://partsregistry.org/Part:BBa_P1010 P1010] EcoR ISpe IInsert682104.917August 01
[http://partsregistry.org/Part:BBa_R0062 R0062] EcoR ISpe IInsert55277.823August 07
[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3305+/-255.122August 07
[http://partsregistry.org/Part:BBa_J23116 J23116] EcoR ISpe IInsert52+/-208.126August 12
[http://partsregistry.org/Part:BBa_K145001 K145001] (T7 poly) EcoR ISpe IInsert2732+/-814.127August 12
[http://partsregistry.org/Part:BBa_F1610 F1610] EcoR IXba IVector3956264.535August 19
[http://partsregistry.org/Part:BBa_K1450151 K145151] EcoR ISpe IInsert3061925.236August 19
[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR IXba IVector13209217.625August 19
[http://partsregistry.org/Part:BBa_I712074 I712074]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert112+/-303.928August 14
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert101+/-304.029August 14
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR ISpe IInsert365+/-206.930August 14
[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR IXba IVector3113+/-208.331August 14
[http://partsregistry.org/Part:BBa_E0022 E0022]+[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector4086+/-3014.832August 14
[http://partsregistry.org/Part:BBa_C0062 C0062]+[http://partsregistry.org/Part:BBa_B0015 B0015] EcoR IXba IVector4080+/-3032.833August 14
[http://partsregistry.org/Part:BBa_C0062 C0062] EcoR ISpe IInsert774285.010August 12
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert144+/-200.918August 22
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert144+/-302.919August 21
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR ISpe IInsert311+/-300.921August 21
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert90+/-282.638August 25
[http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022] EcoR ISpe IInsert311+/-2812.039August 25
[http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_F1610 F1610] XbaI-Vector431+/-525.940August 25
[http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert71+/-282.541August 25
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032] EcoR ISpe IInsert144+/-282.442August 25
[http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoR ISpe IPlasmid20562427.743August 25
[http://partsregistry.org/Part:BBa_K145001 K145001] EcoR ISpe IInsert2730+/-2124.324August 22
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert432+/-304.634August 22
[http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] EcoR ISpe IInsert432+/-303.737August 22

Things stored in freezer in lab (-20°C)

Miscellaneous
Description Name µl left Concentration Box Date
Primer GFP-LVA10849020 µM1August 01
Primer GFP-LVA10859020 µM2August 01
dNTP mixATP, GTP, TTP, CTP7010 mM3August 01
Primer GFP-LVA1084500100 µM4August 01
Primer GFP-LVA1085500100 µM5August 01
Primer Test1074800100 µM6August 07
Primer Test1075780100 µM7August 07
Primer T7-UmuD1076560100 µM8August 07
Primer T7-UmuD1077477100 µM9August 07
Primer T7-UmuD1078467100 µM10August 07
Primer T7-UmuD107945100 µM11August 07
Primer Transduction1097627100 µM12August 07
Primer Transduction1098710100 µM13August 07
Primer Transduction1099608100 µM14August 07
Primer Anti-sense LuxI1100522100 µM15August 07
Primer Anti-sense LuxI1101237100 µM16August 07
Primer Test107410020 µM17August 07
Primer Test107510020 µM18August 07
Primer T7-UmuD107610020 µM19August 07
Primer T7-UmuD107710020 µM20August 07
Primer T7-UmuD107810020 µM21August 07
Primer T7-UmuD107910020 µM22August 07
Primer Transduction109710020 µM23August 07
Primer Transduction109810020 µM24August 07
Primer Transduction109910020 µM25August 07
Primer Anti-sense LuxI110010020 µM26August 07
Primer Anti-sense LuxI110110020 µM27August 07
dNTP mixATP, GTP, TTP, CTP902 mM28August 19
Primer ccdB1127100100 µM29August 19
Primer ccdB1128100100 µM30August 19
Primer K1451501129100100 µM31August 19
Primer K1451501130100100 µM32August 19
Primer ccdB11279020 µM33August 19
Primer ccdB112898020 µM34August 19
Primer K14515011299901 µM35August 19
Primer K14515011309901 µM36August 19
dNTP mixATP, GTP, TTP, CTP9902 µM37August 19