Revision as of 10:50, 27 August 2008

Construction of pFlhB - mRFP Tripart (LVA+)

Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.

Digestion

Measurement of the concentration of D187 purified

Protocol (it's same that for Miniprep)

Digestion Name	Concentration (µg/mL)	Ratio 260/280
D187

Ligation

Protocol

Ligation Name	Vector Name	Volume Vector (µL)	Insert	Volume Insert (µL)
L1	D187		D186
Control L1	D187		-	-

Cloning of EnvZ* in pSB1A2

Transformation results

	control			insert / vector mass ratio
	transformation with pUC19	transformation without plasmid	ligation without insert	1,8 / 1	2,4 / 1	3,1 / 1
number of colonies	many	0	0	8	5	2

@@ Line 51: / Line 51: @@
 {|border="1" style="text-align:center"
-|
+|rowspan="2"|
 |colspan="3"|'''control'''
 |colspan="3"|'''insert / vector mass ratio'''
 |-
-|
 |'''transformation with pUC19'''
 |'''transformation without plasmid'''

Team:Paris/August 27

From 2008.igem.org