Team:Paris/August 27
From 2008.igem.org
(Difference between revisions)
(→Cloning of EnvZ* in pSB1A2) |
(→Cloning of EnvZ* in pSB1A2) |
||
Line 70: | Line 70: | ||
|2 | |2 | ||
|- | |- | ||
+ | |'''number of clones picked up for screening''' | ||
+ | |colspan="3"| | ||
+ | |4 | ||
+ | |2 | ||
+ | |2 | ||
|} | |} |
Revision as of 10:52, 27 August 2008
Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ* in pSB1A2Transformation results
|