Team:Paris/August 27
From 2008.igem.org
(Difference between revisions)
(→Cloning of EnvZ* in pSB1A2) |
(→Cloning of EnvZ* in pSB1A2) |
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*positive control: S158 (pSB3K3) | *positive control: S158 (pSB3K3) | ||
*negative control: no template | *negative control: no template | ||
+ | |||
+ | =Cloning of OmpR*= | ||
+ | ==Digestion== | ||
+ | ===Determination of the concentration of DNA=== | ||
+ | ===Name of the digestions=== | ||
+ | ===Protocol of digestion=== | ||
+ | ===Cleaning of the digestion products=== | ||
+ | ==Ligation== | ||
+ | ===Determination of the concentration of DNA=== | ||
+ | ===Protocol of ligation=== |
Revision as of 12:52, 27 August 2008
Construction of pFlhB - mRFP Tripart (LVA+)Aim : Construction of "pFlhB-RBS-mRFP-dbl ter" (pFlhB-I732078) We can only do the construction with mRFP Tripart (LVA+) because the stable strain with the Biobricks I732011 (mRFP Tripart LVA-) don't to growth.
DigestionMeasurement of the concentration of D187 purifiedProtocol (it's same that for Miniprep)
Ligation
Cloning of EnvZ* in pSB1A2Transformation results
PCR screening
Cloning of OmpR*DigestionDetermination of the concentration of DNAName of the digestionsProtocol of digestionCleaning of the digestion productsLigationDetermination of the concentration of DNAProtocol of ligation |