"

 

From 2008.igem.org

Revision as of 12:48, 2 September 2008 (view source) Yoshimi (Talk | contribs) ← Older edit		Revision as of 12:49, 2 September 2008 (view source) Yoshimi (Talk | contribs) Newer edit →
Line 378:		Line 378:
	{|align="justify"		{|align="justify"
	|[[Image:Chiba-0825-2.JPG]]		|[[Image:Chiba-0825-2.JPG]]
		+	|
		+	From left;
		+	|}
		+
		+	{|align="justify"
		+	|[[Image:Chiba-0825-3.JPG]]
		+	|
		+	From left;
		+	|}
		+	{|align="justify"
		+	|[[Image:Chiba-0825-4.JPG]]
		+	|
		+	From left;
		+	|}
		+	{|align="justify"
		+	|[[Image:Chiba-0825-5.JPG]]
	|		|
	From left;		From left;

---

Revision as of 12:49, 2 September 2008

β班の実験ノート

Contents 1 Week 1 2 Week 2 3 Week 3 4 Week 4

Week 1

• 17 August, 2008

Transformation

• BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
• BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
• BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
• BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
• BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
• BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates

• 18 August, 2008

Transformation

• BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
• BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
• BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
• BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
• BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
• BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
• BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
• BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

--->(20/8)Mini prep

• 19 August, 2008

PCR

• BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
• BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
• BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
• BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
• BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control

--->Gel Check

BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK

• 20 August, 2008

(18/8)--->Mini prep

• BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
• BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
• BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
• BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
• BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
• BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
• BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]

--->Gel Check

Sample No	①	②	③	④
Sample DNA	3	3	3	3
Loading Dye	2	2	2	2
dH2O	7	7	7	7
TOTAL	12	12	12	12

From the left; ①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK ②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK ③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK ④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None

BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK

--->Digestion

• BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
• BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]

• 21 August, 2008

(20/8)--->PCR

• BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
• BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
• BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
• BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]

--->Gel Check

From the left; BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK

Transformation

• BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
• BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
• BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
• BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

• 22 August, 2008

Transformation

• BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
• BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
• BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
• BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]

(Locate & 2 Punch ~iyama ver.~)

• BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
• BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
• BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates

• 23 August, 2008

--->Mini prep

• BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
• BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
• BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
• BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]

--->Digestion

• BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
• BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
• BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
• BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
• BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
• BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

--->Gel Check</table>

From the left; ①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK ②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK ③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK ④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] M BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]

BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion

Week 2

• 24 August, 2008

(22/8)--->Mini prep

• BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
• BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
• BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
• BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

--->Digestion

• BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
• BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
• BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
• BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)

--->Gel Check

Sample No	①	②	③	④	⑤	⑥	⑦	⑧
Sample DNA	1	1	1	1	1	1	1	1
Loading Dye	1	1	1	1	1	1	1	1
dH2O	4	4	4	4	4	4	4	4
TOTAL	6	6	6	6	6	6	6	6

From the left; ①BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007) ②BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) ③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006) ④BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006) （↓single digestuion） ⑤BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007) ⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) ⑦BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006) ⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

Sample No	⑤	⑥	⑦	⑧
Sample DNA	9	9	9	9
Loading Dye	2	2	2	2
dH2O	1	1	1	1
TOTAL	12	12	12	12

From the left; （single digestuion） ⑤BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007) ⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) ⑦BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006) ⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

• 25 August, 2008

(24/8)--->Digestion

• BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
• BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)

--->Gel Check

Sample No	①	②	③	④
Sample DNA	9	9	9	9
Loading Dye	2	2	2	2
dH2O	1	1	1	1
TOTAL	12	12	12	12

From left;

From left;

From left;

From left;

From left;

• 26 August, 2008

--->Gel Check

Sample No	⑤	⑥	⑦	⑧
Sample DNA	9	9	9	9
Loading Dye	2	2	2	2
dH2O	1	1	1	1
TOTAL	12	12	12	12

From left;

• 27 August, 2008

PCR

Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).

• 30 August, 2008

Week 3

• 31 August, 2008

• 1 September, 2008

• 2 September, 2008

• 3 September, 2008

• 4 September, 2008

• 5 September, 2008

• 6 September, 2008

Week 4

• 7 September, 2008

• 8 September, 2008

• 9 September, 2008

• 10 September, 2008

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

• Recent changes
• What links here
• Related changes
• Special pages
• My preferences

• Printable version
• Permanent link
• Privacy policy
• Disclaimers