Team:Chiba/jk/β

From 2008.igem.org

(Difference between revisions)
Line 468: Line 468:
</table>
</table>
-
:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
+
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
Line 505: Line 505:
::: -> OK
::: -> OK
|}
|}
 +
 +
 +
*26 August, 2008
 +
(8/25)--->'''[[Team:Chiba/protocol/ligation/gelex|Gel Extract]]'''
 +
 +
:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]'''
 +
:::①5μl
 +
:::②5μl
 +
:::③27μl
 +
 +
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 +
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No</td>
 +
<td>①</td><td>②</td><td>③</td>
 +
</tr>
 +
<tr>
 +
<td>Sample DNA</td>
 +
<td>2</td><td>2</td><td>2</td>
 +
</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>2</td><td>2</td><td>2</td>
 +
</tr>
 +
<tr>
 +
<td>H2O</td>
 +
<td>8</td><td>8</td><td>8</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>12</td><td>12</td><td>12</td>
 +
</tr>
 +
</table>
 +
{|align="justify"
{|align="justify"
|[[Image:Chiba-0825-5.JPG]]
|[[Image:Chiba-0825-5.JPG]]
|
|
From left;
From left;
 +
::①insert -> OK
 +
::②insert -> OK?
 +
::③Vector -> OK
|}
|}
-
*26 August, 2008
+
 
 +
 
 +
 
:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">

Revision as of 15:36, 3 September 2008

β班の実験ノート

Contents

Week 1

  • 17 August, 2008
Transformation
  • BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
  • BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
  • BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
  • BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
  • BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
  • BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates


  • 18 August, 2008
Transformation
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]


--->(20/8)Mini prep


  • 19 August, 2008
PCR
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
  • BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]・・・control


--->Gel Check
Chiba-0818.JPG
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
  • BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
  • BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
  • BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]・・・control ->OK


  • 20 August, 2008
(18/8)--->Mini prep
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]


--->Gel Check
Sample No
Sample DNA 3333
Loading Dye 2222
dH2O 7777
TOTAL 12121212
Chiba-0820.JPG

From the left;
①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

Chiba-0820-2.JPG
  • BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
  • BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
  • BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
  • BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
Chiba-0820-3.JPG
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
Chiba-0820-4.JPG
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK


--->Digestion
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]


  • 21 August, 2008
(20/8)--->PCR
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]


--->Gel Check
Chiba-0821.JPG

From the left;

  • BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
  • BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
  • BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
  • BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK


Transformation
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]


  • 22 August, 2008
Transformation
  • BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
  • BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
  • BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]
(Locate & 2 Punch ~iyama ver.~)
  • BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
  • BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
  • BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates



  • 23 August, 2008
--->Mini prep
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]


--->Digestion
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]


--->Gel Check</table>
Chiba-0820.JPG

From the left;
①BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
②BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
③BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
④BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK

Chiba-0823-1.JPG
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
Chiba-0823-2.JPG
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
  • M
  • BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
  • BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
  • BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
Chiba-0823-3.JPG
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion
  • BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion


Week 2

  • 24 August, 2008
(22/8)--->Mini prep
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
--->Digestion
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
  • BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
  • BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
--->Gel Check
Sample No
Sample DNA 11111111
Loading Dye 11111111
dH2O 44444444
TOTAL 66666666
Chiba-0824-1.JPG

From the left;

⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)

(↓single digestuion)

⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
Sample No
Sample DNA 9999
Loading Dye 2222
dH2O 1111
TOTAL 12121212
Chiba-0824-2.JPG

From the left; (single digestuion)

⑤BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
⑥BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2006)
⑦BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2007)
⑧BBa_J13002[http://partsregistry.org/Part:BBa_J13002](2006)
  • 25 August, 2008
(24/8)--->Digestion
①Double Digestion:BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
②Double Digesiton:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
③Single Digestion:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
④BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
Sample No
Sample DNA 5511
EcoRⅠ 0.5o.5--
SpeⅠ 0.50.5--
Buffer 1 11--
Buffer 3 --1-
BSA 11--
dH2O 227.5-
TOTAL 1010101
--->Gel Check
Sample No
Sample DNA 10101010
Loading Dye 2221
dH2O ---4
TOTAL 1212126
Chiba-0825-1.JPG

From right;

①Double Digestion:BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007) -> OK
②Double Digesiton:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> ?(low)
③Single Digestion:BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> ?(low)
④BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007) -> OK(low)


)--->Digestion

①BBa_C0178[http://partsregistry.org/Part:BBa_C0178]
②BBa_C0170[http://partsregistry.org/Part:BBa_C0170]
③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
]

</tr>

Sample No
Sample DNA 33ng/μl×60μl33ng/μl×60μl100ng/μl×20μl
PstⅠ 222
XbaⅠ 22-
SpeⅠ --2
Buffer 2 --5
Buffer 3 1010-
BSA 10105
dH2O 161616
TOTAL 10010052
--->Gel Check
Sample No
Sample DNA 303030
Loading Dye 666
TOTAL 363636
Chiba-0825-2.JPG
①BBa_C0178[http://partsregistry.org/Part:BBa_C0178]
Insert:609bp -> OK
Chiba-0825-3.JPG
②BBa_C0170[http://partsregistry.org/Part:BBa_C0170]
Insert:609bp -> OK
Chiba-0825-4.JPG
③BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
-> OK


  • 26 August, 2008

(8/25)--->Gel Extract

--->Zymo Clean
①5μl
②5μl
③27μl
--->Gel Check
Sample No
Sample DNA 222
Loading Dye 222
H2O 888
TOTAL 121212
Chiba-0825-5.JPG

From left;

①insert -> OK
②insert -> OK?
③Vector -> OK



--->Gel Check
Sample No
Sample DNA 9999
Loading Dye 2222
dH2O 1111
TOTAL 12121212
Chiba-0826.JPG

From left;


  • 27 August, 2008
PCR
Colony PCR of 5 colonies from ligation plates (27/8) and one from control plate(BBa_F2620[http://partsregistry.org/Part:BBa_F2620](2007)).
Chiba-0827.JPG

From left;

Chiba-0827-2.JPG

From left;

  • 28 August, 2008]
Chiba-0828.JPG

From left;

  • 29 August, 2008
  • 30 August, 2008

Week 3

  • 31 August, 2008
  • 1 September, 2008
  • 2 September, 2008
  • 3 September, 2008
  • 4 September, 2008
  • 5 September, 2008
  • 6 September, 2008


Week 4

  • 7 September, 2008
  • 8 September, 2008
  • 9 September, 2008
  • 10 September, 2008


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