Team:Paris/August 28
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='''Transformation of OmpR*'''= | ='''Transformation of OmpR*'''= | ||
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- | = | + | =Cloning of EnvZ*= |
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- | + | The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. | |
- | + | ==Digestion== | |
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- | + | [[Image:EnvZ pSB1A2.JPG|thumb|Digestion of EnvZ* and lasR-pSB1A2]] | |
- | [[ | + | |
{|border="1" style="text-align: center" | {|border="1" style="text-align: center" | ||
- | |''' | + | |'''Digestion n°''' |
- | |''' | + | |'''Name''' |
- | |''' | + | |'''volume of DNA digested''' |
- | |''' | + | |'''total volume reaction''' |
- | |''' | + | |'''enzymes used''' |
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- | | | + | |D159 |
- | | | + | |PCR147 (EnvZ*) |
- | | | + | |3 µL |
- | | | + | |30 µL |
- | | | + | |XbaI & PstI |
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|D116 | |D116 | ||
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|MP108 (lasR-pSB1A2) | |MP108 (lasR-pSB1A2) | ||
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|2 µL | |2 µL | ||
- | | | + | |30 µL |
- | | | + | |XbaI & PstI |
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|} | |} | ||
+ | <br> | ||
+ | '''Purification''' | ||
+ | *EnvZ*: directly by the Qiagen kit | ||
+ | *pSB1A2: excision of the band of interest (2 kb) and then purification using the Qiagen kit | ||
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+ | =Freezer empty= | ||
+ | [[Team:Paris/Notebook/Protocols#Digestion |Protocol]] | ||
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+ | We did again [[Team:Paris/Notebook/Freezer#Digestion Products |D110]] and [[Team:Paris/Notebook/Freezer#Digestion Products |D125]]. |
Latest revision as of 19:09, 9 September 2008
Transformation of OmpR*Cloning of EnvZ*The cloning of EnvZ* didn't worked. So we decided to try one more time, starting from the PCR product. Digestion
Freezer empty |