Team:Chiba/jk/γ
From 2008.igem.org
(Difference between revisions)
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<br>TRANSFORMATION | <br>TRANSFORMATION | ||
<br>BBa_J63001(yeast optimized YFP)-->colony | <br>BBa_J63001(yeast optimized YFP)-->colony | ||
+ | *'''2008,8,20(wed)''' | ||
+ | Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620 | ||
+ | :結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620 | ||
+ | ::失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008) | ||
+ | *'''2008,8,21(thu)''' | ||
+ | Lux CDABE(J32007:2007),RBS(B0034:2007) | ||
+ | :結果:成功→RBS | ||
+ | ::失敗→Lux CDABE(二回行ったがどちらもコロニーができなかった)→三回目に成功 | ||
+ | *'''2008,8,22(sun)''' | ||
+ | Venus YFP | ||
+ | :結果 | ||
- | |||
- | |||
== [[Mini prep]] == | == [[Mini prep]] == |
Revision as of 06:21, 14 September 2008
17,August 2008
PCR
BBa_E0430①
BBa_E0420②
BBa_J06702③
BBa_S01416④
BBa_I73003⑤
BBa_I73004⑥
BBa_I730026⑦
BBa_I763002⑧
Sample No | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ |
DNA tamplate | 1 | 1 | 1 | 1 | >1 | 1 | 1 | 1 |
FW primer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
VR primer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
dNTPmix | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
thermo pol buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
dH2O | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
TOTAL | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
20,August 2008
TRANSFORMATION
BBa_J52008(Luciferase Renilla)-->colony
BBa_I712019(Luciferase Fire fly)-->no colony
BBa_J332002(LacZ)-->colony
BBa_E2030(Venus YFP)-->not so good
YFP(P-GEX)-->colony
BBa_F2620-->colony
BBa_T9002-->colony
21,August 2008
TRANSFORMATION
BBa_J32007(Lux CDABE)-->no colony
BBa_B0034(RBS)-->colony
22,August 2008
MINI PREP
BBa_J52008
BBa_F2620
BBa_T9002
24,August 2008
TRANSFORMATION
BBa_J63001(yeast optimized YFP)-->colony
- 2008,8,20(wed)
Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620
- 結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
- 失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
- 2008,8,21(thu)
Lux CDABE(J32007:2007),RBS(B0034:2007)
- 結果:成功→RBS
- 失敗→Lux CDABE(二回行ったがどちらもコロニーができなかった)→三回目に成功
- 2008,8,22(sun)
Venus YFP
- 結果
Mini prep
AHL receiver
IPTG receiver
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