Team:Paris/August 22
From 2008.igem.org
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'''Results''': The clones tested didn't have the insert. | '''Results''': The clones tested didn't have the insert. | ||
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='''Construction of pFlgA - GFP Generator'''= | ='''Construction of pFlgA - GFP Generator'''= | ||
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='''Construction for FIFO'''= | ='''Construction for FIFO'''= | ||
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|L161 | |L161 | ||
- | |D167 (FV) + | + | |D167 (FV) + D132 (FI)<br>pFlgA - YFP tripart (LVA+) |
|Amp | |Amp | ||
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- | =Promoter characterization plasmids= | + | ='''Promoter characterization plasmids'''= |
==Transformation of ligations from August 20th== | ==Transformation of ligations from August 20th== |
Latest revision as of 00:54, 21 September 2008
Analysis of the transformant of FlhDC+promotor
PCRPCR screening programm
Digestiontotal volume reaction (30 µL)
Incubation 2h55 at 37°C and then 20 min at 65°C. Electrophoresis
Results: The clones tested didn't have the insert.
Construction of pFlgA - GFP GeneratorAim : Construction of "pFlgA-RBS-GFP-dbl ter" (pFlgA-E0240) DigestionDigestion
Gel Extraction
Measurement of the concentration of D168 purifiedProtocol (it's same that for Miniprep)
Ligation
Construction for FIFOAim : Construction of pFlgA - YFP tripart (+/- LVA) "pFlgA-RBS-YFP-dbl ter" (pFlgA-E0430/E0432) Transformation of the ligations we did yesterday
Construction for synchronizationTransformation of the ligations we did yesterday
LigationTransformation
Promoter characterization plasmidsTransformation of ligations from August 20thTop 10 cells were used
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