Team:Paris/September 22

From 2008.igem.org

(Difference between revisions)
(→Digestions)
(→Digestions)
Line 8: Line 8:
|'''Plasmid'''
|'''Plasmid'''
|'''Description'''
|'''Description'''
-
|'''Miniprep used'''
+
|'''Miniprep concentration'''
|'''Enzymes'''
|'''Enzymes'''
|'''Size expected'''
|'''Size expected'''
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|MP118
|MP118
|
|
-
|
+
|130
|EX
|EX
|
|
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|MP104
|MP104
|
|
-
|
+
|78
|SP
|SP
|
|
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|MP114
|MP114
|
|
-
|
+
|284
|ES
|ES
|
|
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|MP165
|MP165
|
|
-
|
+
|131
|EX
|EX
|
|
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|MP118
|MP118
|
|
-
|
+
|131
|SP
|SP
|
|
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|MP101
|MP101
|
|
-
|
+
|396
|SP
|SP
|
|
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|MP168
|MP168
|
|
-
|
+
|145
|EX
|EX
|
|
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|MP172
|MP172
|
|
-
|
+
|144
|ES
|ES
|
|
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|MP164
|MP164
|
|
-
|
+
|111
|EX
|EX
|
|
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|MP171
|MP171
|
|
-
|
+
|132
|XP
|XP
|
|
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|MP144
|MP144
|
|
-
|
+
|157
|SP
|SP
|
|
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|MP101
|MP101
|
|
-
|
+
|396
|XP
|XP
|
|
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|MP179
|MP179
|
|
-
|
+
|486
|XP
|XP
|
|
|
|
|}
|}

Revision as of 11:32, 23 September 2008

Digestions

Protocol Digestion

Digestion name Plasmid Description Miniprep concentration Enzymes Size expected Concentration after gel extraction
D125 MP118 130 EX
D161 MP104 78 SP
D162 MP114 284 ES
D166 MP165 131 EX
D179 MP118 131 SP
D180 MP101 396 SP
D187 MP168 145 EX
D194 MP172 144 ES
D198 MP164 111 EX
D200 MP171 132 XP
D204 MP144 157 SP
D205 MP101 396 XP
D206 MP179 486 XP
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