Team:Warsaw/Calendar-Main/19 May 2008

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2. PCR - translation fusion: AID + T7 RNA-polimerase
2. PCR - translation fusion: AID + T7 RNA-polimerase
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Primers: AIDlNrH and T7pXbSal
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Primers:  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and  
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
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Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion
Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion

Revision as of 13:43, 26 September 2008

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1. Gel electrophoresis (yesterday PCR's) and DNA isolation from proper bands.

2. PCR - translation fusion: AID + T7 RNA-polimerase

Primers: AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion

Annealing temperature: 55 °C

Annealing time: 4 minutes

20 cycles

3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)