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Team:Warsaw/Calendar-Main/19 May 2008
From 2008.igem.org
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| + | <p>Michał K.:</p> | ||
| + | <p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">friday</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands.</p> | ||
| - | + | <p>2. PCR - translation fusion: AID + T7 RNA-polimerase</p> | |
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| - | 2. PCR - translation fusion: AID + T7 RNA-polimerase | + | |
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | ||
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Revision as of 17:09, 26 September 2008
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Michał K.: 1. Gel electrophoresis (friday PCR's) and DNA isolation from proper bands. 2. PCR - translation fusion: AID + T7 RNA-polimerase Primers: AIDlNrH and T7pXbSalTemplate DNA: AID for translation fusion and T7 RNA-polimerase for translation fusion Annealing temperature: 55 °C Annealing time: 4 minutes 20 cycles 3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polimerase)
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