Team:Warsaw/Calendar-Main/19 May 2008

From 2008.igem.org

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<p>Michał K.:</p>
<p>Michał K.:</p>
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<p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">friday</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands.</p>
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<p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands.</p>
<p>2. PCR - translation fusion: AID + T7 RNA-polymerase</p>
<p>2. PCR - translation fusion: AID + T7 RNA-polymerase</p>

Revision as of 15:28, 29 September 2008

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Michał K.:

1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands.

2. PCR - translation fusion: AID + T7 RNA-polymerase

Primers:

AIDlNrH and T7pXbSal

Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion

Annealing temperature: 55 °C

Annealing time: 4 minutes

20 cycles

3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)