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Team:Warsaw/Calendar-Main/20 May 2008
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<p>Michał K: <br> | <p>Michał K: <br> | ||
| - | 1. PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).</p> | + | 1.<html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).</p> |
<p>Primers: </p> | <p>Primers: </p> | ||
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2. Gel electrophoresis. <br> | 2. Gel electrophoresis. <br> | ||
| - | 3. Optimalization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles. | + | 3. Optimalization of <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles. |
<p>Primers: </p> | <p>Primers: </p> | ||
Revision as of 22:21, 30 September 2008
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Michał K: Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
35 cycles 2. Gel electrophoresis. Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
4. Gel electrophoresis.
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