Team:Warsaw/Calendar-Main/20 May 2008

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35 cycles <br>
35 cycles <br>
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2. Gel electrophoresis. <br>
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2. Optimalization of <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.
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3. Optimalization of <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.
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<p>Primers: </p>
<p>Primers: </p>
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Annealing time: 4 minutes <br>
Annealing time: 4 minutes <br>
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4.  Gel electrophoresis.
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3.  Gel electrophoresis.

Revision as of 12:57, 1 October 2008

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Michał K:
1.PCR - translation fusion: AID + T7 RNA-polymerase - optymalization (temperature gradient 60°C - 80°C).

Primers:

AIDlNrH and T7pXbSal Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
Annealing time: 4 minutes
35 cycles
2. Optimalization of PCR - translation fusion: AID + T7 RNA-polymerase - MgCl2 cocentration and number of cycles.

Primers:

AIDlNrH and T7pXbSal


Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion


Annealing time: 4 minutes

3. Gel electrophoresis.



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