Piotr:

1. Design of primers for sequencing something we want from pMPM-T5

Primers:

pMPMT5seqF pMPMT5seqR AIDseqP

Michał K.:
1. PCR - translation fusion: AID + T7 RNA-polymerase

Primers:

AIDlNrH and T7pXbSal

Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion

Annealing temperature : 73°C
Annealing time: 4 minutes

20 cycles
2. Gel electrophoresis and isolation of proper bands (3300 bp).