Team:Warsaw/Calendar-Main/3 June 2008
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<h3>Preparation of T7 constructs</h3> | <h3>Preparation of T7 constructs</h3> | ||
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- | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA from proper bands | + | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper bands. </li> |
- | <li>Digestion of DNA with HindIII and SalI.</li> | + | <li>Digestion of DNA with HindIII and SalI (2x Tango buffer).</li> |
<li>Digestion of pMPM-T5 + AID with HindIII and SalI.</li> | <li>Digestion of pMPM-T5 + AID with HindIII and SalI.</li> |
Revision as of 13:43, 1 October 2008
Preparation of T7 constructs
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