Team:Warsaw/Calendar-Main/23 July 2008
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1. DNA fragment of omega_A isolated from gel on 12 July digested with SacI and NotI. <br> | 1. DNA fragment of omega_A isolated from gel on 12 July digested with SacI and NotI. <br> | ||
2. Clean-up of digested DNA fragment. <br> | 2. Clean-up of digested DNA fragment. <br> | ||
- | 3. | + | 3. Digest (SacI and NotI) and dephosphorylation (CIAP) of pACYC177+OmpA_alpha. <br> |
4. Gel electrophoresis and gel-out of 4300 bp band. <br> | 4. Gel electrophoresis and gel-out of 4300 bp band. <br> | ||
5. Ligation of DNA fragments from 2. and 4. (1 hr)<br> | 5. Ligation of DNA fragments from 2. and 4. (1 hr)<br> |
Revision as of 18:01, 1 October 2008