Team:Warsaw/Calendar-Main/3 June 2008
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5 + AID with HindIII and SalI.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5 + AID with HindIII and SalI.</li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up of reaction products.</a></li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up of reaction products.</a></li> | ||
- | <li>Ligation of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li> | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of p.4 products (pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion).</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> TOP10 with ligation product.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> TOP10 with ligation product.</li> | ||
<li>Plating transformants on LB+tetracycline.</li> | <li>Plating transformants on LB+tetracycline.</li> | ||
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<li>DNA gel electrophoresis.</li> | <li>DNA gel electrophoresis.</li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA from proper band.</a></li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA from proper band.</a></li> | ||
- | <li>Ligation of product p.11 (pMPM-T5 + T7 RNA-polymerase).</li> | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of product p.11 (pMPM-T5 + T7 RNA-polymerase).</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> TOP10 with ligation product.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E.coli</i> TOP10 with ligation product.</li> | ||
<li>Plating transformants on LB + tetracycline.</li> | <li>Plating transformants on LB + tetracycline.</li> |
Revision as of 18:39, 1 October 2008
Preparation of T7 constructs
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