Team:Warsaw/Calendar-Main/22 May 2008

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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqR">pMPMT5seqR</a></li>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pMPMT5seqR">pMPMT5seqR</a></li>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
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<h4>Michał K.:</h4>
<h4>Michał K.:</h4>

Revision as of 22:31, 4 October 2008

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Piotr:

  1. Design of primers for sequencing something we want from pMPM-T5.

    2. Primers:

Michał K.:

  1. PCR - translation fusion: AID + T7 RNA-polymerase
    Primers:
    Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
    Annealing temperature : 73°C
    Elongation time: 4 minutes
    20 cycles
  2. Gel electrophoresis and isolation of proper band (3300 bp).

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