"
Team:Warsaw/Calendar-Main/19 May 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
| Line 2: | Line 2: | ||
<!-- do not edit above me! --> | <!-- do not edit above me! --> | ||
<html> | <html> | ||
| - | < | + | <p> |
| + | <ol> | ||
| + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase<br> | ||
| - | < | + | Primers: <br> |
| + | |||
| + | <ul> | ||
| + | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a></li> | ||
| + | <li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></li> | ||
| + | </ul> | ||
| + | |||
| + | |||
| + | |||
| + | Template DNA: purified PCR products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> - AID and T7 RNA-polymerase for translation fusion <br> | ||
| + | |||
| + | Annealing temperature : 73°C <br> | ||
| + | Elongation time: 4 minutes <br> | ||
| + | |||
| + | 20 cycles <br></li> | ||
| + | <li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of proper band (3300 bp).</li></ol></p> | ||
| + | </html> | ||
<html> | <html> | ||
Revision as of 11:12, 5 October 2008
 |
|
||||||||
 |
|
||||||||
Michał L., Ewa, Marcin:1. Overnight inoculation and induction: Top10 + 0,5 mM IPTG pMPM-AID + Glc + 0,01% L-Ara pTrc99a-AID + Glc + 0,5 mM IPTG
|
|
