Team:Warsaw/Calendar-Main/19 May 2008

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(Difference between revisions)
Line 27: Line 27:
<h4>Michał L., Ewa, Marcin:</h4>
<h4>Michał L., Ewa, Marcin:</h4>
-
<p>1. Overnight inoculation and induction:</p>  
+
<ol><li>Overnight inoculation and induction:</li>  
<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> + 0,5 mM IPTG</p>
<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> + 0,5 mM IPTG</p>
<p>pMPM-AID + Glc + 0,01% L-Ara</p>
<p>pMPM-AID + Glc + 0,01% L-Ara</p>

Revision as of 11:13, 5 October 2008

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  1. PCR - translation fusion: AID + T7 RNA-polymerase
    Primers:
    Template DNA: purified PCR products from 16 May - AID and T7 RNA-polymerase for translation fusion
    Annealing temperature : 73°C
    Elongation time: 4 minutes
    20 cycles
  2. Gel electrophoresis and isolation of proper band (3300 bp).

Michał L., Ewa, Marcin:

  1. Overnight inoculation and induction:

    2. Top10 + 0,5 mM IPTG

    pMPM-AID + Glc + 0,01% L-Ara

    pTrc99a-AID + Glc + 0,5 mM IPTG

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