Team:Warsaw/Calendar-Main/22 May 2008

From 2008.igem.org

(Difference between revisions)
Line 14: Line 14:
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDseqP">AIDseqP</a></li></ul>
</p></ol>
</p></ol>
 +
 +
<html>
<h4>Michał K.:</h4>
<h4>Michał K.:</h4>
-
<p>
+
<p><ol>
-
<ol>
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with transcription fusion.</li>
-
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - translation fusion: AID + T7 RNA-polymerase<br>
+
-
 
+
-
Primers: <br>
+
-
 
+
-
<ul>
+
-
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a></li>  
+
-
<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></li>
+
-
</ul>
+
-
 
+
-
 
+
-
Template DNA: purified PCR products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> - AID and T7 RNA-polymerase for translation fusion <br>
+
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion.</li>
-
Annealing temperature : 73&deg;C <br>
+
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).</li>
-
Elongation time: 4 minutes <br>
+
-
20 cycles <br></li>
+
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).</li></html>
-
<li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of proper band (3300 bp).</li></ol></p>
+
-
</html>
+
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 11:43, 5 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Piotr:

  1. Design of primers for sequencing something we want from pMPM-T5.

    2. Primers:

Michał K.:

  1. Isolation of hypothetical pMPM-T5 with transcription fusion.
  2. Isolation of hypothetical pMPM-T5 with translation fusion.
  3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).
  4. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/22_May_2008"