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Team:Warsaw/Calendar-Main/3 June 2008
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<h4>Michał K.:</h4> | <h4>Michał K.:</h4> | ||
<ol> | <ol> | ||
| - | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band. </li> | + | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band. </li></ol> |
| - | + | <h4>Piotr:</h4> | |
| + | <ol> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5 + transcription fusion with EcoRHI and HindIII.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPM-T5 + transcription fusion with EcoRHI and HindIII.</li> | ||
<li>Use of T4 polymerase for blunting.</li> | <li>Use of T4 polymerase for blunting.</li> | ||
<li>DNA gel electrophoresis.</li> | <li>DNA gel electrophoresis.</li> | ||
| - | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">Isolation of DNA</a> from proper band.</li> |
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of product p.11 (pMPM-T5 + T7 RNA-polymerase).</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of product p.11 (pMPM-T5 + T7 RNA-polymerase).</li> | ||
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li> | ||
Revision as of 17:56, 5 October 2008
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Preparation of pMPMT5-AID+AIDT7 constructMichał K.:
Piotr:
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