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Team:Warsaw/Calendar-Main/3 June 2008
From 2008.igem.org
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<h4>Michał K.:</h4> | <h4>Michał K.:</h4> | ||
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| - | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band. </li></ol> | + | <li>DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band (3300 bp). </li></ol><br> |
<h3>Preparation of pMPMT5+T7 construct</h3> | <h3>Preparation of pMPMT5+T7 construct</h3> | ||
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<ol> | <ol> | ||
| - | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of | + | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digest</a> of pMPMT5 + transcription fusion with EcoRHI and HindIII (2x Tango buffer).</li> |
<li>Use of T4 polymerase for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting"> blunting</a>.</li> | <li>Use of T4 polymerase for <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#blunting"> blunting</a>.</li> | ||
<li>DNA gel electrophoresis.</li> | <li>DNA gel electrophoresis.</li> | ||
Revision as of 18:02, 5 October 2008
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Preparation of pMPMT5-AID+AIDT7 constructMichał K.:
Preparation of pMPMT5+T7 constructPiotr:
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