Team:Warsaw/Calendar-Main/21 August 2008

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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin
<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin
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<li>Inoculated to liquid LB with ampicillin pET15b+OmpA-alfa</li>
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<li>Inoculation to liquid LB with ampicillin: pET15b+OmpA-alfa</li>
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Revision as of 21:37, 8 October 2008

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Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A Primers used: AP+NotI and AL+SacI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin
  3. Inoculation to liquid LB with ampicillin: pET15b+OmpA-alfa

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