Team:Bologna/Notebook

From 2008.igem.org

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(ā†’Protocols)
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{|  
{|  
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|width=45| '''NĀ°'''
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| '''Protocol'''
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|width=90| '''Igem Code'''
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| '''Safety level'''
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|width=470| '''Fragment'''
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|- align="center"
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|width=90| '''Plasmid'''
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| * [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]]
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|width=110| '''Length (bp)'''
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| 1
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|width=90| '''Vitality'''
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|}
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|width=45| '''Fluo'''
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|* [[Team:Bologna/Protocols#Amplification|Amplification]]
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|- style="background:#f6efcd; color:grey"
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* [[Team:Bologna/Protocols#Plates_preparation|Plates preparation]]
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* [[Team:Bologna/Protocols#Amplification|Amplification]]
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* [[Team:Bologna/Protocols#Transformation|Transformation]]
* [[Team:Bologna/Protocols#Transformation|Transformation]]
* [[Team:Bologna/Protocols#Inoculation|Inoculation]]
* [[Team:Bologna/Protocols#Inoculation|Inoculation]]

Revision as of 13:42, 9 October 2008

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HOME THE PROJECT THE TEAM PARTS SUBMITTED TO THE REGISTRY MODELING NOTEBOOK BIOSAFETY


Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.

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Contents

Protocols

Protocol Safety level
* Plates preparation 1

|* Amplification

Week 1: from 07/21/08 to 07/27/08

  1. Enzymatic digestion of: C0051 E\S, LACI* X\P.
  2. Gel preparation , electrophoretic run.
  3. Amplification of: TETR , PUV, PRECA.


Week 2: from 07/28/08 to 08/03/08

  1. Transformation of 2008 registry plasmides: I714930 e I714927 (ceppo TOP10)
  2. Ligation of: R0051 + S0100-T ; J04500 + C0051; R0082 + E0240


Week 3: from 08/04/08 to 08/10/08