Team:Warsaw/Calendar-Main/3 June 2008
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- | <h3>Preparation of pMPMT5+T7 construct< | + | <h3>Preparation of pMPMT5+T7 construct</h3> |
- | Piotr:</ | + | <h4>Piotr:</h4> |
<p><ol> | <p><ol> | ||
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li> | <li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemotransform">Transformation</a> of <i>E.coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> with ligation product.</li> | ||
<li>Plating transformants on LB + tetracycline.</li> | <li>Plating transformants on LB + tetracycline.</li> | ||
- | </ol></p> | + | </ol></p><br> |
Revision as of 10:08, 11 October 2008
Preparation of pMPMT5-AID+AIDT7 constructMichał K.DNA (PCR products from previous day) gel electrophoresis and isolation of DNA from proper band (20 cycles - 3300 bp). Fig. 1. Preparation of pMPMT5+T7 constructPiotr:
Fig. 1. PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct. Lane 1 - 25 cycles, lane 2 - 20 cycles. |