Team:Warsaw/Calendar-Main/3 June 2008
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DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band (20 cycles - 3300 bp). Fig. 1. </p> | DNA (PCR products from previous day) gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> of DNA from proper band (20 cycles - 3300 bp). Fig. 1. </p> | ||
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<h3>Preparation of pMPMT5+T7 construct</h3> | <h3>Preparation of pMPMT5+T7 construct</h3> | ||
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Revision as of 10:10, 11 October 2008
Preparation of pMPMT5-AID+AIDT7 constructMichał K.DNA (PCR products from previous day) gel electrophoresis and isolation of DNA from proper band (20 cycles - 3300 bp). Fig. 1. Preparation of pMPMT5+T7 constructPiotr
Fig. 1. PCR results of translational fusion for the pMPMT5-AID+AIDT7 construct. Lane 1 - 25 cycles, lane 2 - 20 cycles. |