Team:Warsaw/Calendar-Main/12 July 2008
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+ | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | ||
+ | <p><ol> | ||
+ | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of Geneart_Z and pACYC177+OmpA_omega with SacI and NotI (pACYC177 was also dephosphorylated with CIAP)(3 hr).</li> | ||
+ | <li> Gel electrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands 220 bp (for Geneart_Z lane)and 4050 bp (pACYC177+OmpA_omega lane). </li> | ||
+ | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pACYC177+OmpA_omega and Z (1 hr). </li> | ||
+ | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation. </li> | ||
+ | <li> Transformants plating on LB + kanamycin.</li> | ||
+ | </ol> | ||
+ | </p> | ||
+ | |||
<h3>Preparation of constructs with OmpA protein fusions and Cloning of protein Z DNA to OmpA constructs</h3> | <h3>Preparation of constructs with OmpA protein fusions and Cloning of protein Z DNA to OmpA constructs</h3> |
Revision as of 14:48, 11 October 2008
Cloning of protein Z DNA to OmpA constructs
Preparation of constructs with OmpA protein fusions and Cloning of protein Z DNA to OmpA constructs
Polymerase Chain Ligation on linker-A and omega-linkerMichał L., Ewa, Marcin
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