Team:Warsaw/Calendar-Main/12 July 2008
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmid DNA</a> from cultures inocluated on previous day. </li> | ||
- | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI | + | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmid with SacI and NotI (BamHI buffer) - 2 h.</li> |
- | <li> Gel electrophoresis of digested DNA</li> | + | <li> Gel electrophoresis of digested DNA.</li> |
- | <li> We are glad to find the proper clone | + | <li> We are glad to find the proper clone and inoculate liquid LB with ampicillin to freeze bacteria carrying pKS-A construct.</li></ol> |
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Revision as of 15:22, 11 October 2008
Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of construct pKS with A proteinMichał L., Ewa, Marcin
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