Team:Warsaw/Calendar-Main/23 July 2008

From 2008.igem.org

(Difference between revisions)
Line 6: Line 6:
-
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3>h4>Paweł</h4>
+
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA</h3><h4>Paweł</h4>
<ol><li>Results of ligation: 6 colonies grown.</li>
<ol><li>Results of ligation: 6 colonies grown.</li>
<li>Each colony cultured overnight in LB + ampicillin.</li></ol>
<li>Each colony cultured overnight in LB + ampicillin.</li></ol>

Revision as of 19:35, 11 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 



Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Isolation of plasmids from cultures inocluated on previous day
  2. Control digest of isolated plasmids with NdeI and NotI. Two positives obtained (proper band: ~150 bp visible)
  3. One of positive plasmids transformed into TOP10 and plated on LB+amp, overnight, for further isolation of pET15b+Z+omega vector

Antoni

  1. Setup of overnight culture E. coli TOP10 (LB broth) for preparation of chemocompetent bacteria.

Cloning of protein A DNA to OmpA constructs

Michał K.

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
  2. Control digest of isolated plasmids with BamHI and SacI.